Publications

2007

Blanca G., Delagoutte E., Tanguy-Le Gac N., Johnson N.P., Baldacci G. and Villani G. (2006). Accessory proteins assist exonuclease-deficient bacteriophage T4 DNA Polymerase in replicating past an abasic site. Biochem. J., 402(2), 321-9. 

Crespan E., Alexandrova L., Khandazhiskaya A., Jasko M., Kukhanova M., Villani G., Hubscher U., Spadari S. and Maga G. (2007). Expanding the repertoire of DNA polymerases substrates : template-instructed incorporation of non-nucleoside triphosphates by DNA polymerases b and l. Nucleic Acids Res., 35(1), 45-57. 

Gembka A., Toueille M., Smirnova E., Poltz R., Ferrari E., Villani G. and Hübscher U. (2007). The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair. Nucleic Acids Res., 35(8), 2596-608. 

Maga G., Villani G., Crespan E., Wimmer U., Ferrari E., Bertocci B. and Hübscher U. (2007). 8-oxo-guanine bypass by human DNA polymerases in the presence of auxiliary proteins. Nature, 447(7144), 606-8.

2006

 Datta K., Johnson N.P. and Von Hippel P.H. (2006). Mapping the conformation of the nucleic acid framework of the T7 RNA polymerase elongation complex in solution using low-energy CD and fluorescence spectroscopy. J. Mol. Biol., 360, 800-813.

 Maga G., Shevelev I., Villani G., Spadari S. and Hübscher U. (2006). Human replication protein A can suppress the intrinsic in vitro mutator phenotype of human DNA polymerase lambda. Nucleic Acids Res., 34(5), 1405-1415.

 Nimonkar A.V., Tanguy-Le Gac N., Villani G. and Boehmer P.E. (2006). Escherichia coli RecA promotes strand invasion with cisplatin-damaged DNA. Biochimie, 88(5), 535-542.

2005

Lesca C., Germanier M., Raynaud-Messina B.,  Pichereaux C., Etievant C.,  Emond S., Burlet-Schiltz O., Monsarrat B.,  Wright M. and  Defais M. (2005) "DNA Damage INDUCE Gamma-tubulin-Rad51 nuclear complexes in mammalian cells", Oncogene, 24, 5165-5172.

Johnson, N.P., Baase, W.A. et Von Hippel, P.H. (2005) Investigating local conformations of double-stranded DNA by low energy circular dichroism of pyrrolo-cytosine., Proc. Natl. Acad, Sci, USA 102, 7169-7173.

Oliva Trastoy M., Defais M. and Larminat F. (2005) “Resistance to the antibiotic zeocin by stable expressionof the Sh ble gene does not fully suppress DNA cleavage induced by zeocin” Mutagenesis, 20, 111-114.

Johnson N.P., Baase W.A., von Hippel P.H. (2005) "Low-energy CD of RNA hairpin unveils a loop conformation required for lambda N antitermination activity". J. Biol. Chem. Jul 19 on line

Locatelli G.A., Di Santo R., Crespan E., Costi R., Roux A., Hübscher U., Shevelev I., Blanca G., Villani G., Spadari S., Maga G. (2005) "Diketo Hexenoic Acid Derivatives Are Novel Selective Non-Nucleoside Inhibitors of Mammalian Terminal Deoxynucleotidyl Transferases, with Potent Cytotoxic Effect against Leukemic Cells". Mol Pharmacol., 68, 538-550

Crespan E, Zanoli S, Khandazhinskaya A, Shevelev I, Jasko M, Alexandrova L, KuKhanova M, Blanca G, Villani G, Hübscher U, Spadari S, Maga G (2005) "Incorporation of non-nucleoside triphosphate analogues opposite to an abasic site by human DNA polymerases beta and lambda" . Nucleic Acids Res., 33 (13), 4117-427

2004

M.E.Arana, L.Song, N.Tanguy Le Gac, D.Parish, G.Villani and P.E.Boehmer (2004) “On the role of proofreading exonuclease in bypass of a 1,2(GpG) cisplatin adduct by the herpes simplex virus-1 DNA” DNA Repair., 3, 659-669.

Larminat F., Germanier M., Papouli E. and Defais M. (2004) "Impairment of homologous recombination control in the Fanconi Anemia-like Chinese Hamster mutant VH4", Biol. Cell, 96, 545-552.

G.Maga, G.Blanca,I.Shevelev,I.Frouin,K.Ramadan,S.Spadari ,G.Villani and U.Hubscher (2004) "The human DNA polymerase lambda interacts with PCNA through a domain important for DNA primer binding and the interaction is inhibited by p21/WAF1/CIP1" FASEB Journal, 18, 1743-1745.

N.Tanguy Le Gac, E. Delagoutte, M. Germain and G.Villani (2004) “Inactivation of the 3’-5’exonuclease of the replicative T4 DNA polymerase allows translesion DNA synthesis at an abasic site” J. Mol. Biol., 336, 1023-1034.

Johnson NP, Baase WA, Von Hippel PH. (2004) "Low-energy circular dichroism of 2-aminopurine dinucleotide as a probe of local conformation of DNA and RNA". Proc Natl Acad Sci U S A. 101: 3426-3431.

2003

Defais M., Phez E. and Johnson N.P. (2003). Kinetic mechanism for the formation of the presynaptic complex of the bacterial recombinase RecA. J. Biol. Chem., 278, 3545-3551.

Blanca G., Shevelev I., Ramadan C.,Villani G., Spadari S., Hubscher U. and Maga G. (2003). Human DNA polymerase  diverged in evolution from DNA polymerase  towards specific Mn ++ dependence : a kinetic and thermodynamic study. Biochemistry, 42, 7467-7476.

Maga G., Villani G., Ramadan K., Shevelev I., Tanguy Le Gac N., Blanco L., Blanca G., Spadari S. and Hübscher U. (2003). Human DNA polymerase  functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis. J. Biol. Chem., 277, 48434-48440.

Ramadan K., Maga G., Shevelev I.,Villani G., Blanco L. and Hubscher U. (2003). Human DNA polymerase  possesses terminal deoxyribonucletidyl transferase activity and can elongate RNA primers : implications for novel functions. J. Mol. Biol., 328, 63-72.

Shevelev I., Blanca G., Villani G., Ramadan. K., Spadari.S., Hubscher.U. and Maga G. (2003). Mutagenesis of human DNA polymerase  : essential roles of Tyr 505 and Phe 506 for both DNA polymerase and terminal transferase activities. Nucleic Acids Res., 31, 6916-6925.

Boehmer P.E. and Villani G. (2003). Herpes simplex virus type-1 : a model for genome transactions. Prog. Nucleic Acid Res., 75, 139-171.

2002

Larminat F., Germanier M., Papouli E. and Defais M. (2002). deficiency in BRACA2 leads to overall increase in homologous recombination. Oncogene, 21, 5188-5192.

Papouli E., Defais M. and Larminat F. (2002). Overexpression of metallothionein-II sensitizes hamster cells to DNA cross-linking agent-mediated apoptosis through inhibition of NF-B activation. J. Biol. Chem., 277, 4764-4769.

Villani G., Tanguy Le Gac N., Wasangu L., Burnouf D., Fuchs R.P. and Boehmer P. (2002). Effect of Manganese on in vitro replicationof damaged DNA catalysed by Herpes simplex virus type-1 DNA polymerase. Nucleic Acids Res., 30, 3323-3332.

Pascucci B., Maga G., Hubscher U., Bjoras M., Seeberg E., Hickson I.D., Villani G., Giordano C., Cellai L. and Dogliotti E. (2002 ). Reconstitution of the base excision repair pathway for 7,8-dihydro-8-oxoguanine with purified human proteins. Nucleic Acids Res., 30(10), 2124-2130.

Tanguy Le Gac N. and Boehmer PE. (2002). Activation of the herpes simplex virus type-1 origin-binding protein (UL9) by heat shock proteins. J Biol Chem., 277, 5660-566.

2001

Arana M.E., Haq B., Tanguy Le Gac N. and Boehmer P.E. (2001). « Modulation of the Herpes Simplex Virus Type-1 UL9 DNA helicase by its cognate single-strand DNA-binding protein, ICP8 », J. Biol. Chem., 276, 6840-6845.

Arnaudeau C., Rozier L., Cazaux C., Defais M., Jenssen D. and Helleday T. (2001). Rad51 supports spontaneous non-homologous recombination in mammalian cells, but not the corresponding process induced by topoisomerase inhibitors, Nucleic Acids Res., 29, 662-667.

Gourves A.S., Defais M. and Johnson N. (2001). Equilibrium binding of single-stranded DNA to the secondary DNA binding site of a recombinase-proficient RecA filament. J. Biol. Chem., 276, 9613-9619.

Maga G., Villani G., Tillement V., Stucki M., Locatelli G.A., Frouin I., Spadari S. and Hübscher U. (2001). « Okazaki fragment processing : Modulation of the strand displacement activity of DNA polymerase  by the concerted action of replication protein A, proliferating cell nuclear antigen and flap endonuclease-1 » Proc. Natl. Acad. Sci. USA, 98, 14298-303.

Papouli E., Defais M. and Larminat F. (2001). « Overexpression of metallothionein-II sensitizes hamster cells to DNA coss-linking agent-mediated apoptosis through inhibition of NF-B activation » J. Biol. Chem., 277, 4764-4769.

Defais M. and Devoret R. (2001). « SOS responses » in Encyclopedia of life Sciences, Macmillan Reference limited, Grove’s Dictionaries Inc. (version web).

2000

Papouli E, Lafon C, Valette A, Zdzienicka MZ, Defais M, Larminat F. (2000). "Involvement of apoptosis in mitomycin C hypersensitivity of Chinese hamster cell mutants". Biochem. Pharmacol., 59(9):1101-1107.

Tanguy Le Gac N., Hoffmann J.-S., Hindges R. and Villani G. (2000). DNA synthesis by CHO cell extracts on fork-like DNA templates containing the major cisplastin adduct requires a ligation step. Biochimie, 82, 41-49.

Villani G. and Tanguy Le Gac N. (2000). Effect of damage on DNA helicases activity in vitro and possible biological consequences. J. Biol. Chem., 275, 33185-33188.

Germanier M., Defais M., Bohr V. A. and Larminat F. (2000). Transcription-coupled repair is inducible in hamster cells. Nucleic Acids Res., 28, 4674-4678.

Gourves A.S., Tanguy Le Gac N., Villani G., Boehmer P. and Johnson N. (2000). « Equilibrium binding of single stranded DNA with HSV-1 coded single stranded DNA binding protein, ICP8. » J. Biol. Chem., 2000, 275, 10864-10869.

1999

Bastide L., Boehmer P.E., Villani G. and Lebleu B. (1999) « Inhibition of a DNA-helicase by peptide nucleic acids » Nucleic Acids Res. 27, 551-554.

Villani G., Tanguy Le Gac N. and Hoffmann J.S. (1999) « Replication of platinated DNA and its mutagenic consequences » in « Cisplatin : Chemistry and Biochemistry of a leading Anticancer Drug » ed. M. Volkan Kisakürek, Verlag, Chimica Acta , Zürich.