Publications

2007

Simeone R., Constant P., Malaga W., Guilhot C., Daffé M. and Chalut C. (2007). Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis. FEBS lett., 274(8), 1957-69. 

Simeone R., Constant P., Guilhot C., Daffé M. and Chalut C. (2007). Identification of the missing trans-acting enoyl reductase required for phthiocerol dimycocerosates and phenolglycolipids biosynthesis in M. tuberculosis. J. Bacteriol., 189, 4597-4602. 

Sacco E., Legendre V., Laval F., Zerbib D., Montrozier H., Eynard N., Guilhot C., Daffé M., and Quemard A. Rv3389C from Mycobacterium tuberculosis, a member of the (R)-specific hydratase/dehydratase family. Biochim Biophys Acta., 1774(2), 303-11. 

Guilhot C. and Daffé M. (2007). Polyketides and polyketides-containing glycolipids of M. tuberculosis: structure, biosynthesis and biological activities. In S.H.E. Kaufmann & E. J. Rubin (eds), Tuberculosis: Molecular Biology and Biochemistry, Vol. 1 Wiley-Vch Verlag GmBH & Co. KGaA, Weinheim. (in press). 

Guilhot C., Chalut C. and Daffé M. (2007). Biosynthesis and roles of phenolic glycolipids and related molecules in M. tuberculosis. In M. Daffé & J.-M. Reyrat (eds), The mycobacterial cell envelope : an overview”. ASM press. Washington, D.C. Sous presse. 

Portevin, D., Malaga, W. and Guilhot C. (2007) The use of temperature sensitive plasmid in mycobacteria. In T. Parish & A. Brown (eds.), Methods in Molecular Biology, Vol. : Mycobacteria Protocols. Humana Press Inc., Totowa, N.J.

2006

Chalut C., Botella L., de Sousa-D’Auria C., Houssin C. and Guilhot C. (2006). The non redundant roles of two 4’-phosphopantheinyl transferases in vital processes of Mycobacteria. Proc. Natl. Acad. Sci. USA, 103, 8511-8516.

2005

Portevin D., de Souza-D’Auria C., Montrozier H., Houssin Ch., Stella A., Lanéelle MA., Bardou F., Guilhot C. and Daffé M. (2005). Evidence that the activation of substrates used for the condensation leading to the synthesis of Mycolic acids required the acyl-AMP ligase FadD32 and an AccD4-containing Acyl-CoA carboxylase. J. Biol. Chem., 280(10), 8862-74.

Brevet : « Use of a 4’-Phopshopantetheinyl Transferases as a target for identifying antibiotic molecules », Brevet du CNRS, déposé en décembre 2005, auteurs : Chalut, C., Guilhot, C.

2004

Pérez E., Constant P., Lemassu A., Laval F., Daffé M. and Guilhot C. (2004). Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. J. Biol. Chem., 279 (41), 42574-42583.

 Pérez E., Constant P., Laval F., Lemassu A., Lanéelle M.-A., Daffé M. and C. Guilhot (2004). Molecular dissection of the role of two methyltransferases in the biosynthesis of phenolglycolipids and phthiocerol dimycosertosate in the Mycobacterium tuberculosis complex. J. Biol. Chem., 279 (41), 42584-42592.

Portevin D., de Sousa-D’Auria C., Houssin C., Grimaldi C., Chami M., Daffé M. and Guilhot C. (2004). A polyketide synthase catalyzes the last condensation step of mycolic acid biosynthesis in mycobacteria and related organisms. PNAS, 101, 314-319.

2003

Malaga W., Perez E. and Guilhot C. (2003). Production of unmarked mutations in mycobacteria using site-specific recombination. FEMS Microbiol. Lett., 219, 261-268.

2002

Constant P, Perez E., Malaga W., Lanéelle M.A., Saurel O., Daffé M. and Guilhot C. (2002). Role of the pks15/1 gene in the biosynthesis of phenolglycolipids in the M. tuberculosis complex : evidence that all strains synthesize glycosylated p-hydroxybenzoic methyl esters and that strains devoid of this glycolipid harbour a frameshift mutation in the pks15/1 gene. J. Biol. Chem., 277, 38148-38158.

Puech V., Guilhot C., Perez E., Tropis M., Armitige L.Y., Gicquel B. and Daffé M. (2002). Evidence For A Partial Redundancy of The Fibronecting-binding proteins For The Transfer of Mycoloyl residues onto the Cell Wall Arabinogalactan termini of Mycobacterium tuberculosis. Mol. Microbiol., 44, 1109-1122.

Raynaud C., Guilhot C., Rauzier J., Bordat Y., Pelicic V., Manganelli R., Smith I., Gicquel B.and Jackson M. (2002). Phospholipases C are involved in the virulence of Mycobacterium tuberculosis. Mol. Microbiol., 45, 203-217.

2001

Camacho L. R., Constant P., Raynaud C., Lanéelle M. A., Triccas J. A., Gicquel B., Daffé M. and Guilhot C. (2001). Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculoses evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem., 276, 19845 19854.

Perez E., Samper S., Bordat Y., Guilhot C., Gicquel B. and Martin C. (2001). An essential role for phoP in Mycobacterium tuberculosis virulence, Mol. Microbiol., 41, 179-187.

Jackson M., Camacho L. R., Gicquel B. and Guilhot C. (2001). Gene replacement and transposon delivery using the negative selection marker sacB. In: « Methods in Molecular Biology: Mycobacterium tuberculosis protocols ». Editors: T. Parish & N. Stocker. Humana Press Inc., Totowa N.J., 1-17.

Brevets :

Guilhot, C., Daffé, M., Houssin, C., Portevin, D. et de Sousa, C. Utilisation de la protéine Pks13 codant pour la condensase des acides mycoliques des mycobactéries et genres apparentés comme cible d'antibiotiques. Déposé le 4 Septembre 2003 sous le N°: 0310470.