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Dr. Alain Roussel - Camelid nanobodies: versatile tools for molecular and structural biology studies

 

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Alain Roussel

AFMB, CNRS and Aix-Marseille University, Marseille

Camelid nanobodies: versatile tools for molecular and structural biology studies

In recent years, the use of single-domain camelid immunoglobulins, termed vHHs or nanobodies, has seen increasing growth in biotechnology, pharmaceutical applications and structure/function research. The usefulness of nanobodies in molecular and structural biology is now firmly established whether to solve 3D structures or to perform functional studies. After describing our platform dedicated to the selection and production of nanobodies, I will present the recent results we have obtained in the structural studies of bacterial secretion systems (T2SS, T6SS and T9SS). Finally I will present our current work on the development of neutralizing nanobodies against Ebola virus.

Selected publications

  • Desmyter et al. (2015) Camelid nanobodies: killing two birds with one stone. Curr Opin Struct Biol 32C, 1-8
  • Douzi et al. (2017) Unraveling the self-assembly of the Pseudomonas aeruginosa XcpQ secretin periplasmic domain provides new molecular insights into type II secretion system secreton architecture and dynamics. MBio 8: pii: e01185-17
  • Nguyen et al. (2017) Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol 2: 17103
  • Leone et al. (2018) Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space. Nat Commun 9: 429

Contact: Lionel Mourey (lionel.mourey@ipbs.fr)

Note for visitors: Please come with a valid identity card

14 Jan

11:00 - 12:00

Seminar room - IPBS - Campus 205