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Dr. Guy Lippens - Fluxomics by NMR (or how bacteria reproduce themselves)

 

Guy Lippens

Toulouse Biotechnology Institute (TBI), Toulouse

Fluxomics by NMR (or how bacteria reproduce themselves)

Measuring the carbon isotopic content of different metabolites is a powerful tool to quantify fluxes in living systems. Spanning the different forms of life, the approach has found applications in the study of bacteria, yeast, plants, or humans. In the latter case, the altered metabolism of cancer cells has attracted significant interest.

Here, we will present our efforts to develop NMR methodology to study the central metabolism of bacteria. Using both the 15N and 13C nuclei as an editing tool for characterization of the different metabolites,  we will use amino acid biosynthesis as an example to illustrate the power of the method. We finally will detail an improved NMR workflow aimed at assigning the high-resolution sub-spectrum of the phospho-metabolites in a complex mixture.

Selected references

  • P. Millard, E. Cahoreau, M. Heuillet, J.-C. Portais, G. Lippens, Anal Chem 2017, 89, 2101–2106
  • D. Sinnaeve, M. Dinclaux, E. Cahoreau, P. Millard, J.-C. Portais, F. Létisse, G. Lippens, Anal Chem 2018, 90, 4025–4031
  • G. Lippens, E. Cahoreau, P. Millard, C. Charlier, J. Lopez, X. Hanoulle, J. C. Portais, The Analyst 2018, 143, 620–629
  • M. Dinclaux, E. Cahoreau, P. Millard, F. Létisse, G. Lippens, Magn Reson Chem 2020, DOI 10.1002/mrc.4988
  • S. M. Prud’homme, Y. M. I. Hani, N. Cox, G. Lippens, J.-M. Nuzillard, A. Geffard, Metabolites 2020, 10, DOI 10.3390/metabo10060256

Contact: Olivier Neyrolles (Olivier.Neyrolles@ipbs.fr) & Andrew Atkinson (Andrew.Atkinson@ipbs.fr)

Link to attend the seminar: https://meet.starleaf.com/4104682445/app

19 Jan

11:00 - 12:00

Online seminar