My research interests are focused on using mass spectrometry as a tool for studying soluble and membrane associated protein assemblies.
During my time as a Ph.D. student I applied mass spectrometry to unravel the structural mechanism of activation of the NADPH oxidase from neutrophils. The project also gave me the opportunity to gain experimental experience in various biophysical techniques as well as in molecular biology, protein expression and purification. I expanded and developed these skills during my two postdoctoral positions in Oxford and Strasbourg, by applying them to significantly larger soluble complexes and membrane proteins. I obtained, in 2015, a CNRS Research Associate position at the Institute of Pharmacology and Structural Biology in Toulouse, where I am currently developing structural MS-based methods for the analysis of supramolecular complexes, including the 20S human proteasome and its regulators.
Researcher at CNRS (French National Center for Scientific Research) at the Institute of Pharmacology and Structural Biology (IPBS-UMR5089), 205 route de Narbonne, 31077 Toulouse cedex 04, FRANCE
Education - Training
- 2003-2006 : Biochemistry Engineer of the National Institute of Applied Sciences in Lyon
- 2005-2006 : Master in Structural and Functional Biochemistry of the University Claude Bernard Lyon 1 / 6-month placement in a Mexican Oceanographic Centrer (Ensenada, Baja California, Mexico) to study the production and analysis of superoxide dismutase extracted from microalgae cultivated in a photo-bioreactor
- 2006-2010 : PhD in Structural Biology of the University Joseph Fourier of Grenoble (France), Laboratory : Institute of Stuctural Biology (Dr Eric Forest and Prof. Franck Fieschi) Subject: Structural and functional analysis of neutrophil NADPH oxidase: use of mass spectrometry to characterize p47phox conformational changes upon activation
- 2011-2014 : Postdoctoral fellow at the University of Oxford (Chemistry Departement) (UK) – Laboratory : Physical and Theoretical Chemistry Laboratory (Prof. Dame Carol Robinson) - Subject : Use of native MS and Ion-Mobility to characterize soluble and membrane protein complexes.
- 2014-2015 : Postdoctoral fellow at University of Strasbourg (France) - Laboratory : BioOrganic Mass Spectrometry Laboratory (Dr Sarah Sanglier-Cianférani) - Subject : Use of structural MS to investigate monoclonal antibodies and other protein complexes.
- 2015 : Permanent researcher position at CNRS
- My scientific achievements consist of 35 research articles, accumulating a citation index of 1044 with an h-index of 18, according to
Main selected publications (Top 10 since 2010):
- Marcoux J, Man P, Petit-Haertlein I, Vives C, Forest E and Fieschi F (2010) “p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex” Journal of Biological Chemistry 285 (37) : 28980-28990.
- Marcoux J*, Wang S*, Politis A, Reading E, Ma J, Biggins P, Zhou M, Tao H, Zhang Q, Chang G, Morgner N, Robinson CV (2013) “Mass spectrometry reveals synergistic binding of nucleotides, lipids and drugs to a multidrug resistance efflux pump” Proceedings of the National Academy of Sciences 110(24) : 9704-9709.
- Marcoux J and Robinson CV (2013) “Twenty years of gas phase structural biology” Structure 21(9) : 1541-1550.
- Marcoux J, Politis A, Marshall D, Rinehart D, Wallace MI, Tamm LK, Robinson CV (2014) “A new model for full length OmpA: native mass spectrometry reveals partial dimerization via C-ter domain” Structure 22(5) : 781-790.
- Marcoux J, Cianférani S (2015) “Towards integrative structural mass spectrometry: benefits from hybrid approaches” Methods 89:4-12.
- Marcoux J, Mangione PP, Porcari R, Degiacomi M, Verona G, Taylor GW, Giorgetti S, Raimondi S, Sanglier-Cianférani S, Benesch JL, Cecconi C, Naqvi MM, Gillmore JD, Hawkins PN, Stoppini M, Robinson CV, Pepys MB, Bellotti V (2015) “A novel mechano-enzymatic cleavage mechanism underlies transthyretin amyloidogenesis” EMBO Molecular Medicine 7(10) : 1337-1349
- Mehmood S, Marcoux J, Gault J, Quigley A, Michaelis S, Young SG, Carpenter EP, Robinson CV (2016) “Mass spectrometry captures off-target drug binding and provides mechanistic insights into the human metalloprotease ZMPSTE24” Nature Chemistry 8(12) : 1152-1158
- Parra J*, Marcoux J*, Poncin I, Canaan S, Herrmann JL, Nigou J, Burlet-Schiltz O, Rivière M (2017) “Scrutiny of Mycobacterium tuberculosis 19kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm” Scientific Reports 7:43682
- Carel C*, Marcoux J*, Parra J, Réat V, Latgé G, Laval F, Burlet-Schiltz O, Demange P, Milon A, Daffé M, Tropis M, Renault M (2017) “Post-translational O-mycoloylation mediates protein targeting to the mycomembrane in C. glutamicum” Proceedings of the National Academy of Sciences 114(16) : 4231-4236
- Locard-Paulet M, Parra J, Albigot R, Mouton-Barbosa E, Bardi L, Burlet-Schiltz O, Marcoux J (2018) “VisioProt-MS: interactive 2D maps from intact protein mass spectrometry” BioInformatics In Press doi: 10.1093/bioinformatics/bty680
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- Frédéric Rivière / Master 2 Student (2015)
- Julien Parra / Post-Doctoral Fellow (2015-2018)
- Efthymia Ioanna Koufogeorgou / Master 2 Student (2015-2016)
- Dusan Zivkovic / Master 2 Student (2016-2017)
- Jean Lesne / Master 2 Student (2017-2018)
- Dusan Zivkovic / PhD Student (2017-2020)
- Sandrine Lise / Master 2 Student (2018/2019)