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Julien Marcoux is awarded the CNRS bronze Medal

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The CNRS bronze Medal recognizes the first work of a promising researcher in his or her field.

 

Julien Marcoux’s research interests are focused towards using mass spectrometry as a tool for studying soluble and membrane associated protein assemblies. As a PhD student Julien applied mass spectrometry to unravel the structural mechanism of activation of the NADPH oxidase [1]. He expanded and developed these skills during two postdoctoral positions in Oxford (Carol Robinson’s group) and Strasbourg (Sarah Cianferani’s group), by applying them to soluble complexes [2] and membrane proteins [3]. His pioneering use of native MS to monitor the binding of detergent, small drugs and lipids to membrane proteins [4] is now widely applied.
Recruited in 2015 as CNRS Research Associate at the Institute of Pharmacology and Structural Biology in Toulouse, he took advantage of the proteomics infrastructure led by Odile Schiltz, to implement and develop structural MS-based methods for the analysis of supramolecular complexes. Top-down proteomics, applied to lipo(glycol)proteins from actinobacteria revealed the existence of unsuspected proteoforms [5,6]. The group also developed several software for structural MS data visualization.
Julien’s main focus is now to decipher the molecular mechanisms underlying the regulation of the different human 20S proteasomes by various regulators. This project, recently granted by an ANR JCJC funding, using Hydrogen-Deuterium exchange coupled to Mass Spectrometry, should rationalize how a given subtype of 20S proteasome recruits certain regulator (unpublished results), and pave the way to more specific and less deleterious proteasome inhibition avenues.

 

More information (in french)

Selected publications

[1] Marcoux J, Man P, Petit-Haertlein I, Vives C, Forest E and Fieschi F (2010) “p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex” Journal of Biological Chemistry 285 (37) : 28980-28990.
[2] Marcoux J, Mangione PP, Porcari R, Degiacomi M, Verona G, Taylor GW, Giorgetti S, Raimondi S, Sanglier-Cianférani S, Benesch JL, Cecconi C, Naqvi MM, Gillmore JD, Hawkins PN, Stoppini M, Robinson CV, Pepys MB, Bellotti V (2015) “A novel mechano-enzymatic cleavage mechanism underlies transthyretin amyloidogenesis” EMBO Molecular Medicine 7(10) : 1337-1349
[3] Marcoux J, Politis A, Marshall D, Rinehart D, Wallace MI, Tamm LK, Robinson CV (2014) “A new model for full length OmpA: native mass spectrometry reveals partial dimerization via C-ter domain” Structure 22(5) : 781-790.
[4] Marcoux J*, Wang S*, Politis A, Reading E, Ma J, Biggins P, Zhou M, Tao H, Zhang Q, Chang G, Morgner N, Robinson CV (2013) “Mass spectrometry reveals synergistic binding of nucleotides, lipids and drugs to a multidrug resistance efflux pump” Proceedings of the National Academy of Sciences USA 110(24) : 9704-9709.
[5] Parra J*, Marcoux J*, Poncin I, Canaan S, Herrmann JL, Nigou J, Burlet-Schiltz O, Rivière M (2017) “Scrutiny of Mycobacterium tuberculosis 19kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm” Scientific Reports 7:43682
[6] Carel C*, Marcoux J*, Parra J, Réat V, Latgé G, Laval F, Burlet-Schiltz O, Demange P, Milon A, Daffé M, Tropis M, Renault M (2017) “Post-translational O-mycoloylation mediates protein targeting to the mycomembrane in C. glutamicumProceedings of the National Academy of Sciences USA 114(16) : 4231-4236