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Phagocyte migration and differentiation

Isabelle Maridonneau-Parini

Group Leader

Macrophages infiltrate all body tissues which are heterogeneous in composition, stiffness and architecture. This process, critical for immune protection, also promotes inflammatory diseases and cancers, and its control has become a pharmacological challenge. In this attempt, we are investigating the molecular and cellular mechanisms involved in macrophage 3D migration.

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We have shown that macrophages use two migration strategies, the amoeboid and mesenchymal modes, depending on the matrix porosity, and that, amongst leukocytes, only macrophages are capable of using the mesenchymal migration mode.

Macrophage infiltration into tumor cell spheroids and in vivo into mouse tumors involves mesenchymal migration.

In the tuberculosis context, macrophages with a bacilli-permissive phenotype exhibit an enhanced capacity for mesenchymal migration. 

Mesenchymal migration requires proteases to create paths in the matrix and podosomes, the unique adhesion structures formed by macrophages. We have identified three podosome effectors, Hck, Filamin A and WASp, as key regulators of mesenchymal migration. An ongoing comparative transcriptomic analysis of 3D-migrating macrophages will lead to the identification of additional candidate genes regulating mesenchymal migration.
As podosomes play a critical role in mesenchymal migration, we have also started investigating their mechanical properties, and have revealed that protrusive forces are generated at podosomes in order to sense the rigidity of the environment. We are currently developing new experimental approaches as well as automated image analysis tools dedicated to the characterization of podosome force, architecture and dynamics. HIV-1, the pathogen responsible for AIDS, uses macrophages as host cells. It manipulates their migration by affecting podosomes, further supporting our team’s strategy of identifying pharmacological targets to control macrophage migration in pathological situations.

 

Main Publications

Souriant S et al. (2019). Tuberculosis boosts HIV-1 production by macrophages through IL-10/STAT3-dependent tunneling nanotube formation. Cell Rep 26(13):3586-99

 

Raynaud-Messina B et al. (2018). The bone degradation machinery of osteoclasts: a novel HIV-1 target that contributes to bone loss. Proc Natl Acad Sci USA 115(11):2556-65

 

Gui P et al. (2018). The protease-dependent mesenchymal migration of tumor-associated macrophages is a novel target in cancer immunotherapy. Cancer Immunol Res 6(11):1337-51

 

Desvignes E et al. (2018). Nanoscale forces during confined cell migration. Nano Lett 10;18(10):6326-33

 

Bouissou A et al. (2017). Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring. ACS Nano 11(4):4028-40

 

Verollet C et al. (2015). HIV-1 reprograms the migration of macrophages. Blood 25:611-22

 

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Scanning electron micrography of human monocyte-derived macrophages (pink) that have infiltrated a thick layer of Matrigel® (grey) for 72h. 
They degrade the extracellular matrix and dig tunnels.

© Renaud Poincloux

 

Scientists

Isabelle Maridonneau-Parini

Isabelle Maridonneau-Parini

INSERM Research director

Fabrice Dumas

Associate Professor - University of Toulouse
Arnaud Labrousse

Arnaud Labrousse

Associate Professor in Cellular Biology - University of Toulouse

Frédéric Lagarrigue

CNRS Research associate in immunology

Véronique Le Cabec

CNRS Research associate

Brigitte Raynaud-Messina

CNRS Research associate

Research Assistants

Myriam Ben Neji

AI CNRS

Postdoctoral fellows

Javier Rey Barroso

Fellow of Human Frontier Science program

Margot Tertrais

Fellow of the Agence nationale de recherche sur le sida et les hépatites virales

PhD Students

Claire Bigot

Fellow of 80'prime program

Rémi Mascarau

Fellow of Toulouse Cancer Santé Fondation

Perrine Verdys

Fellow of Toulouse University

Ophélie Dufrançais

Fellow of Toulouse University

 

2020

Mascarau R, Bertrand F, Labrousse A, Gennero I, Poincloux R, Maridonneau-Parini I, Raynaud-Messina R, Vérollet C. HIV-1-infected human macrophages, by secreting RANKL, contribute to enhanced osteoclastogenesis. BioRxiv

Dupont M, Souriant S, Balboa L, Phong T, Pingris K, Rousset S, Cougoule C, Romboust Y, Poincloux R, Ben Neji M, Allers C, Kaushal D, Kuroda MJ, Benet S, Martinez-Picado J, Izquierdo-Useros N, del Carmen Sasiain M, Maridonneau-Parini I*, Neyrolles O*, Lugo-Villarino G*, Verollet V*. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages. BioRxiv

Accarias S, Sanchez T, Labrousse A, Ben-Neji M, Boyance A, Poincloux R, Maridonneau-Parini I, Le Cabec V*. Genetic engineering of hoxb8 immortalized hematopoietic progenitors: a potent tool to study macrophage tissue migration. J. Cell Science (2020). in press

Mangeat T, Labouesse S, Allain M, Martin E, Poincloux R, Bouissou A, Cantaloube S, Courtaux E, Vega E, Li T, Guénolé A, Rouvière C, Allard S, Campo N, Suzanne M, Wang X, Michaux G, Pinot M, Le Borgne R, Tournier S, Idier J, Sentenac A. Super-resolved live-cell imaging using Random Illumination Microscopy. BioRxiv

 

2019

Souriant S*, Dupont M*, Neyrolles O, Maridonneau-Parini I, Lugo-Villarino G, Vérollet C. Tunneling nanotube formation in HIV-1-infected human macrophages: building bridges for efficient HIV-1 dissemination during co-infection with Mycobacterium tuberculosis. Med Sci (Paris) (2019) 35: 825–827

Xie M, Leroy H, Mascarau R,  Woottum M, Dupont M, Ciccone C, Schmitt A, Raynaud-Messina B, Vérollet V, Bouchet J, Benichou S*, Bracq L*. Cell-to-cell spreading of HIV-1 in myeloid target cells escapes SAMHD1 restriction. mBio (2019) 10(6)

van den Dries K.*, Linder S.*, Maridonneau-Parini I.*, Poincloux R.*. Probing the mechanical landscape: new developments in podosome architecture and mechanics. J. Cell Science (2019) 13;132(24)

Lugo-Villarino G, Cougoule C, Meunier E, Rombouts Y, Vérollet C, Balboa L. The mononuclear phagocyte system revisited: current views in infectious disease. Frontiers in Immunology (2019) Editorial. 10:1443

Raynaud-Messina B, Vérollet C, Maridonneau-Parini I. The osteoclast, a target cell for microorganisms. Bone (2019) 127:315-323

Souriant S, Balboa L, Pingris K, Kviatcovsky D, Cougoule C, Lastrucci C, Bah A, Gasser R, Poincloux R, Raynaud-Messina B, Al Saati T, Inwentarz S, Poggi S, Moraña EJ, Gonzalez-Montaner P, Corti M, Lagane B, Vergne I, Allers C, Kaushal D, Kuroda MJ, Sasiain MDC, Neyrolles O*, Maridonneau-Parini I*, Lugo-Villarino G*, Vérollet C*.  Tuberculosis boosts HIV-1 production by macrophages through IL-10/STAT3-dependent tunneling nanotube formation. Cell Rep. (2019) 26(13):3586-3599

 

2018

Colado A, Genoula M, Cougoule C, Marín Franco JL, Almejún MB, Risnik D, Kviatcovsky D, Podaza E, Elías EE, Fuentes F, Maridonneau-Parini I, Bezares FR, Fernandez Grecco H, Cabrejo M, Jancic C, Sasiain MDC, Giordano M, Gamberale R, Balboa L, Borge M. Effect of the BTK inhibitor ibrutinib on macrophage- and γδ T cell-mediated response against Mycobacterium tuberculosis. Blood Cancer J. (2018) 8(11):100

Desvignes E, Bouissou A, Laborde A, Mangeat T, Proag A, Vieu C, Thibault C, Maridonneau-Parini I*, Poincloux R*. Nanoscale forces during confined cell migration. Nano Letters (2018) 10;18(10):6326-6333

Gui P, Ben-Neji M, Belozertseva E, Dalenc F, Franchet C, Gilhodes J, Labrousse A, Bellard E, Golzio M, Poincloux  R, Maridonneau-Parini I*, Le Cabec V*. The protease-dependent mesenchymal migration of tumor-associated macrophages is a novel target in cancer immunotherapy. Cancer Immunol. Res. (2018) 6(11):1337-1351

Baschieri F, Dayot S, Elkhatib N, Ly N, Capmany A, Schauer K, Betz T, Matic Vignjevic D, Poincloux R, Montagnac G. Clathrin-coated plaques are frustrated endocytosis structures that assemble in response to stiff substrates and serve as signalling platforms to promote sustained Erk activity and cell proliferation. Nature Communications (2018) 9(1):3825

Lugo-Villarino G, Troegeler A, Balboa L, Lastrucci C, Duval C, Mercier I, Benard A, Capilla F, Al Saati T, Poincloux R, Kondova I, Verreck F, Cougoule C, Maridonneau-Parini I, Sasiain MC, Neyrolles O. The C-type lectin receptor DC-SIGN has an anti-inflammatory role in human M(IL-4) macrophages in response to Mycobacterium tuberculosis. Front. Immunol. (2018) 9:1123

Gonçalves-De-Albuquerque CF, Rohwedder I, Ribeiro Silva A, Silveira Ferreira A, Kurz ARM, Cougoule C, Klapproth S, Eggersmann T, Dutra Silva J, Pena de Oliveira G, Luiza Capelozzi V, Gutfilen Schlesinger G, Rijo Costa E, de Cassia Elias Estrela Marins R, Mocsai A, Maridonneau-Parini I, Walzog B, Rocco PRM, Sperandio M, Caire Castro-Faria-Neto H. The yin and yang of tyrosine kinase inhibition during experimental polymicrobial sepsis. Front. Immunol. (2018) 9:901

Cougoule C, Lastrucci C, Guiet R, Mascarau R, Meunier E, Lugo-Villarino G, Neyrolles O, Poincloux R*, Maridonneau-Parini I*. Podosomes, but not the maturation status, determine the protease dependent 3D migration in human dendritic cells. Front. Immunol. (2018) 9:846

Bouissou A, Proag A, Portes M, Soldan V, Balor S, Thibault C, Vieu C, Maridonneau-Parini I*, Poincloux R*. Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions. JoVE (2018) 16;(136)

Genoula M, Marín-Franco JL, Dupont M, Kviatcovsky D, Milillo A, Schierloh P, José Moraña E, Poggi S, Palmero D, Mata-Espinosa D, González-Domínguez E, León-Contreras JC, Barrionuevo P, Rearte B, Córdoba Moreno MO, Fontanals A, Crotta Asis A, Gago G, Cougoule C, Neyrolles O, Maridonneau-Parini I, Sánchez-Torres C, Hernández-Pando R, Vérollet C, Lugo-Villarino G, Sasiain MC, Balboa L. Formation of Foamy Macrophages by Tuberculous Pleural Effusions is triggered by the IL-10/STAT3 Axis through ACAT up-regulation. Front. Immunol. (2018) 9:459

Métais A, Lamsoul I, Melet A, Uttenweiler-Joseph S, Poincloux R, Stefanovic S, Valière A, Stella A, Burlet-Schiltz O, Zaffran S, Lutz PG, Moog-Lutz C. The Asb2α-Filamin A axis is essential for actin cytoskeleton remodeling during heart development. Circulation Research (2018) 122(6):e34-e48

Raynaud-Messina B, Bracq L, Dupont M, Souriant S, Usmani SM, Proag A, Pingris K, Soldan V, Thibault C, Capilla F, Al Saati T, Gennero I, Jurdic P, Jolicoeur P, Davignon JL, Mempel TR, Benichou S, Maridonneau-Parini I*, Verollet C*. The bone degradation machinery of osteoclasts:a novel HIV-1 target that contributes to bone loss. PNAS USA (2018) 115(11):E2556-E2565

Dupont M, Souriant S, Lugo-Villarino G, Maridonneau-Parini I*, Vérollet C*. Tunneling nanotubes: intimate communication between myeloid cells. Front. Immunol. (2018) 9:43

Cervero P, Wiesner C, Bouissou A, Poincloux R, Linder S. Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. Nature Communications (2018) 9(1):515

Lopez-Lopez N, Ramos Martinez AG, Garcia-Hernandez M, Hernandez-Pando R, Castañeda-Delgado J, Lugo-Villarino G, Cougoule C., Neyrolles O, Rivas-Santiago B, Valtierra-Alvarado M, Rubio-Caceres M, Enciso-Moreno J, Serrano CJ. Type-2 Diabetes alters the basal phenotype of human macrophages and diminishes their capacity to respond, internalise and control Mycobacterium tuberculosis. Memórias do Instituto Oswaldo Cruz. (2018) 113(4):e170326

 

2017

Bouissou A, Proag A, Bourg N, Pingris K, Cabriel C, Balor S, Mangeat T, Thibault C, Vieu C, Dupuis G, Fort E, Lévêque-Fort S, Maridonneau-Parini I, Poincloux R. Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring. ACS Nano (2017) PMID:28355484

 

Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, Vu Manh TP, Capilla F, Poincloux R, Pingris K, Nigou J, Rademann J, Dalod MY, Verreck FAW, Al Saati T, Lugo-Villarino G, Lepenies B, Hudrisier D, Neyrolles O. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. PNAS USA (2017) PMID:28069953

 

Negash A, Mangeat T, Labouesse S, Giovannini H, Belkebir K, Chaumet P, Sandeau N, Poincloux R, Bouissou A, Sentenac A. Super-resolution using speckle illumination microscopy. Mathematics in Imaging Conference paper.  Imaging and Applied Optics (2017) MTh1C. 2

 

Demy DL, Tauzin M, Lancino M, Le Cabec V, Redd M, Murayama E, Maridonneau-Parini I, Trede N, Herbomel P.  Trim33 / Tif1-γ is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues.  J Cell Sci. (2017) 130(17):2797-2807

 

2016

Proag ABouissou A, Vieu C, Maridonneau-Parini IPoincloux R. Evaluation of the force and spatial dynamics of macrophage podosomes by multi-particle tracking. Methods (2016) PMID:26342257

2015

Lastrucci C, Bénard A, Balboa L, Pingris KSouriant SPoincloux R, Al Saati T, Rasolofo V, González-Montaner P, Inwentarz S, Moraña E, Kondova I, Verreck FAW, Sasiain MC, Neyrolles O*, Maridonneau-Parini I,*, Lugo-Villarino G and Cougoule C. Tuberculosis is associated with expansion of a motile, permissive and immunomodulatory CD16+ monocyte population via the IL-10/STAT3 axis. Cell Research(2015) PMID:26482950

 

Vérollet C, Le Cabec V, Maridoneau-Parini I. HIV-1-infection of T lymphocytes and macrophages affects their migration via Nef. Front. Immunol. (2015) PMID:26500651

 

Vérollet C, Souriant S, Raynaud-Messina B, Maridonneau-Parini I. HIV1 drives the migration of macrophages. Med Sci (2015)PMID:26340831

 

Proag ABouissou A, Mangeat T, Voituriez R, Delobelle P, Thibault C, Vieu C, Maridonneau-Parini IPoincloux R. Working together: Spatial synchrony in the force and actin dynamics of podosome first neighbors. ACS Nano (2015) PMID: 25791988

 

Medina I, Cougoule C, Drechsler M, Bermudez B, Koenen RR, Sluimer J, Wolfs I, Döring Y, Herias V, Gijbels M, Bot I, de Jager S, Weber C, Cleutjens J, van Berkel TJ, Sikkink KJ, Mócsai A, Maridonneau-Parini I, Soehnlein O, Biessen EA. Hck/Fgr kinase deficiency reduces plaque growth and stability by blunting monocyte recruitment and intraplaque motility. Circulation (2015) PMID: 26068045

 

Alvarez-Zarate J, Matlung HL, Matozaki T, Kuijpers TW, Maridonneau-Parini I, van den Berg TK. Regulation of Phagocyte Migration by Signal Regulatory Protein-Alpha Signaling. PLoS One (2015) PMID:26057870

 

Balboa L, Barrios-Payan J, González-Domínguez E, Lastrucci C, Lugo-Villarino G, Mata-Espinoza D, Schierloh P, Kviatcovsky D, Neyrolles O, Maridonneau-Parini I, Sánchez-Torres C, Sasiain MD, Hernández-Pando R. Diverging biological roles among human monocyte subsets in the context of tuberculosis infection. Clin Sci (Lond) (2015) PMID:25858460

 

Lastrucci C, Baillif V, Behar A, Al Saati T,  Dubourdeau M, Maridonneau-Parini I and Cougoule C. Molecular and cellular profiles of the resolution phase in DAMP-mediated peritonitis and revelation of leukocyte persistence in peritoneal tissues. Faseb J (2015) PMID: 25609430

 

Verollet C, Souriant S, Bonnaud E, Jolicoeur P, Raynaud-Messina B, Kinnaer C, Fourquaux I, Imle A, Benichou S, Fackler O, Poincloux R, Maridonneau-Parini I. HIV-1 reprograms the migration of macrophages. Blood (2015) PMID: 25527710

 

2014

Labernadie ABouissou A, Delobelle P, Balor S, Voituriez R, Proag A, Fourquaux I, Thibault C, Vieu C, Poincloux R*Charrière GM* andMaridonneau-Parini I*. Protrusion Force Microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes . Nature Communications (2014) PMID:25385672

 

Maridonneau-Parini I. Control of macrophage 3D migration: a therapeutic challenge to limit tissue infiltration. Immunol Rev(2014)PMID:25319337

 

Gui PLabrousse AVan Goethem E, Besson A, Maridonneau-Parini ILe Cabec V. Rho/ROCK pathway inhibition by CDK inhibitor p27kip1 participates in the onset of macrophage 3D-mesenchymal migration. J. Cell Sci(2014) PMID:25015295

 

Troegeler A, Lastrucci C, Duval C, Tanne A, Cougoule CMaridonneau-Parini I, Neyrolles O and Lugo-Villarino G. An efficient siRNA-mediated gene silencing in primary human monocytes, dendritic cells & macrophages. Immulogy & Cell Biology (2014) PMID:24890643

 

Maridonneau-Parini I. Podosomes are disrupted in PAPA syndrome. Inside Blood. Blood (2014) PMID:24764557

 

Wiesner C, Le-Cabec V, El Azzouzi K, Maridonneau-Parini I, Linder S. Macrophage migration and matric degradation in 2D and 3D settings. Cell Adhesion and Migration (2014) PMID:24713854

 

Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, Guerquin-Kern JL, Schnappinger D, Sório de Carvalho LP, Poquet Y, Neyrolles O. Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resists acid stress during infection. PLoS Pathog. (2014) PMID:24586151

 

Park H, Dovas A, Hanna S, Lastrucci CCougoule CGuiet RMaridonneau-Parini I, Cox D. Tyrosine phosphorylation of WASP by Hck regulates macrophage function. J Biol Chem. (2014) PMID:24482227

 

2013

Ben Amara A, Gorvel L, Baulan K, Derain-Court J, Buffat C, Vérollet C, Textoris J, Ghigo E, Bretelle F, Maridonneau-Parini I, Mege JL. Placental macrophages are impaired in chorioamnionitis, an infectious pathology of the placenta. J Immunol. (2013) PMID:24163411

 

Vérollet C, Gallois A, Dacquin R, Lastrucci C, Pandruvada S, Ortega N, Poincloux RBehar BCougoule C, Lowell C, Al Saati T, Jurdic P and Maridonneau-Parini I. Hck contributes to bone homeostasis by controlling the recruitment of osteoclast precursors FASEB J. (2013) PMID: 23742809

 

Bochet L, Lehuédé C, Dauvillier S, Wang YY, Dirat B, Laurent V, Dray C, Guiet RMaridonneau-Parini I, Le Gonidec S, Couderc B, Escourrou G, Valet P, Muller C. Adipocyte-derived fibroblasts promote tumor progression and contribute to the desmoplastic reaction in breast cancer. Cancer Res. (2013) PMID:23903958

 

Marchetti M, Capela D, Poincloux R, Benmeradi N, Auriac MC, Le Ru A, Maridonneau-Parini I, Batut J, Masson-Boivin C. Queusosine biosynthesis is required for sinorhizobium meliloti-induced cytoskeletal modifications on HeLa cells and symbiosis with Medicago truncatula. PLoS One. (2013) PMID:23409119

 

2012

Bouchet J, Vérollet C, Herate C, Guenzel C, Jarviluoma A, Mazzolini J, Guenzel C, Rafie S, Chames P, Baty D, Saksela K, Niedergang N,Maridonneau-Parini I and Benichou S. Single-domain antibody-SH3 fusions for efficient neutralization of HIV-1 Nef functions. J. Virol. (2012) PMID: 22345475

 

Maridonneau-Parini I and Cougoule C. Mechanisms of macrophage migration in 3D-environments.Encyplopedia of Inflammatory Diseases Springer Edition (2012)

 

Cougoule CVan Goethem ELe Cabec V, Lafouresse F, Dupré L, Mehraj V, Mège JL, Lastrucci CMaridonneau-Parini I. Blood leukocytes and macrophages of various phenotypes have distinct abilities to form podosomes and migrate in 3D environments. Eur. J. Cell Biol. (2012) PMID: 22999511

 

Guiet RVérollet C, Lamsoul I, Cougoule CPoincloux RLabrousse A, Calderwood DA, Glogauer M, Lutz PG, Maridonneau-Parini I. Macrophage mesenchymal migration requires podosome stabilization by Filamin A. J. Biol. Chem. (2012) PMID:22334688

 

Ariel A, Maridonneau-Parini I, Rovere-Querini P, Levine JS, Mühl H. Macrophages in inflammation and its resolution. Front Immunol. (2012) PMID:23125842

 

2011

Villarino GC, Vérollet CMaridonneau-Parini I and Neyrolles O. Macrophage polarization: convergence point targeted by Mycobacterium tuberculosis and HIV. Front. Immun. (2011) PMID: 22566833

 

Labrousse AMeunier ERecord JLabernadie A, Beduer A, Vieu C, Ben Safta T and Maridonneau-Parini I. Frustrated phagocytosis on micro-patterned immune complexes to characterize lysosome movements in live macrophages. Front. Immun. (2011) PMID: 22566841

 

Le Cabec VVan Goethem EGuiet R and Maridonneau-Parini I. La migration des phagocytes : tour d’horizon. Médecine et Sciences (2011) PMID: 22192751

 

Vérollet C, Charrière GMLabrousse ACougoule CLe Cabec V and Maridonneau-Parini I. Extracellular proteolysis in macrophage migration: Losing grip for a breakthrough. Eur. J. Immunol.(2011) PMID: 21953638

 

Guiet RVan Goethem ECougoule C, Balor S, Valette A, Al Saati T, Lowell CA, Le Cabec V and Maridonneau-Parini I. The process of macrophage migration promotes matrix metalloproteinase-independent invasion by tumour cells. J. Immunol. (2011) PMID: 21880978

 

Van Goethem EGuiet R, Balor S, Charrière GM, Poincloux RLabrousse AMaridonneau-Parini I and Le Cabec V. Macrophage podosomes go 3D. Eur. J. Cell Biol. (2011) PMID: 20801545

 

Botella H, Peyron P, Levillain F, Poincloux R, Poquet Y, Brandli I, Wang C, Tailleux L, Tilleul S, Charrière GM, Waddell SJ, Foti M, Lugo-Villarino G, Gao Q, Maridonneau-Parini I, Butcher PD, Castagnoli PR, Gicquel B, de Chastellier C, Neyrolles O. Mycobacterial p(1)-type ATPases mediate resistance to zinc poisoning in human macrophages. Cell Host Microbe. 2011 PMID: 21925112

 

2010

Labernadie A, Thibault C, Vieu C, Maridonneau-Parini I and Charriere GM. Dynamics of podosome stiffness revealed by atomic force microscopy. Proc.Natl. Acad. Sci USA (2010) PMID: 21081699

 

Vérollet CZhang Y-MLe Cabec V, Mazzolini J, Charrière GLabrousse A, Bouchet J, Medina I, Biessen E, Niedergang F, Benichou S, andMaridonneau-Parini I. HIV-1 Nef triggers the formation of multinucleated giant macrophages in a Hck and protease-dependent manner. J. Immunol (2010) PMID: 20488787

 

Cougoule CLe Cabec VPoincloux R, Saati TA, Mège JL, Tabouret G, Lowell CA, Laviolette-Malirat N and Maridonneau-Parini I. Three-dimensional migration of macrophages requires Hck for podosome organization and extracellular matrix proteolysis. Blood (2010) PMID: 19897576 
Highlighted in Research highlights, Nature Rev Mol Cell Biol (2010) 11(4):231

 

Van Goethem EPoincloux R, Gauffre F, Maridonneau-Parini I and Le Cabec V. Matrix architecture dictates 3D migration modes of human macrophages: differential involvement of proteases and podosome-like structures. J. Immunol. (2010) PMID: 20018633

 

... click here for a complete list of publications.

Collaborations

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Current funding

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03/2020

2020Fred

 

 

 

07/2019
Frédéric Lagarrigue was awarded the "Premier Prix Jeune Chercheur" from the Fonroga Foundation !

 

 

 

05/2019

May 2019_Physics today

 

 

 

04/2019

Our article (Souriant S et al., 2019) published in @CellReports was highlighted in Editor's Choice in @sciencemagazine (April 26th, issue #6438). Great boost for the paper and the health issue we are addressing: co-infection with tuberculosis and AIDS !

 

2019_Science

 

04/2019

2019 La Depeche

 

 

 

03/2019

Press_Shanti

 

 

 

12/2018