Our core facility offers the instrumentation and expertize to visualize complex systems from molecules to whole organisms and at a time scale ranging from nanoseconds to several days by the use of time-lapse imaging. Moreover, it possesses the instrumentation and expertize for the phenotypic characterization and sorting of eukaryotic and prokaryotic cells by flow cytometry.
Our instruments are housed either in standard or Biosafety Level 3 (BSL3) conditions to permit the work with a large nature of samples, including virulent human pathogens.
Access to the core facility is opened to academic as well as private research laboratories without geographical, thematic or institutional restriction. The access to the facility is possible through full service (experiments performed by facility’s engineers), as a fee for hour of equipment usage or as a R&D collaboration to setup new or customised models and/or analyses. Our facility is certified ISO 9001:2008 and NFX 50-900 and integrates the regional platform TRI-Genotoul.
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Implementation and adaption of Instrumentation for Biosafety level 3 microscopy
Multiphoton microscopy (MPM) has revealed important insights into cell-cell interactions and dynamic behavior of cells in vivo, which is due to its ability to provide four-dimensional (x, y, z and time) imaging of tissues over depths of several hundreds of microns while limiting phototoxicity and photobleaching. However, the use of MPM in the context of infections by BSL3 microorganisms that are associated with serious/lethal disease in humans, in particular those transmitted by aerosols, remains a significant challenge. In collaboration with Eurobioconcept and Laviosion Biotech we have designed and validated a bio-confinement strategy to permit the use of MPM in BSL3 settings while respecting biosafety regulations. For this we employed a two-pronged approach for biosafety confinement of the MPM setup. First, we built a custom-made Class III Biosafety Cabinet (BSC) under negative pressure to confine the infectious material being imaged and to prevent any contamination from reaching the other parts of the microscope. Second, we confined the laser components within a modified Class I BSC under positive pressure with HEPA filtered air, thus preventing any contamination to reach any of its parts, even in the case of a leak on the class III BSC or an incident within the room. Our strategy is essential to unlock the use of MPM approaches in BSL3 settings and will help driving in vivo imaging studies of human pathogens.
Barlerin D, Bessière G, Domingues J, Schuette M, Feuillet C, Peixoto A. (2017) Biosafety Level 3 setup for multiphoton microscopy in vivo. Sci Rep. Apr 3;7(1):571. doi: 10.1038/s41598-017-00702-x. ... more information
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