Immunomodulation By Mycobacterial Lipids and Glycoconjugates

We use a multidisciplinary approach combining lipidomics, synthetic chemistry, microbiology, cell biology and animal experimentation to explore at the molecular level several facets of the host-pathogen interaction. Moreover,  we wish to translate this fundamental knowledge into new therapeutic or preventive strategies/tools to be explored 

Modulation of innate immune responses. 
Mtb inhibits innate immune responses, including inflammation and autophagy, thus favoring its survival inside the infected host. Our goals are to uncover the molecular bases of Mtb recognition by pattern-recognition receptors, including TLRs and C-type lectins, and to identify Mtb factors that modulate macrophage function. Moreover, we wish to characterize Mt lipid trafficking within extracellular vesicles and the remodeling of Mtb lipidome during infection.

Glycolipid antigen presentation to CD1-restricted T cells.
Lipids and glycolipids are important antigens that induce CD1-restricted T cell-mediated specific immune responses. We intend to define the repertoire of Mtb lipid T cell epitopes, to characterize the molecular steps and mechanisms of their presentation by antigen-presenting cells and to evaluate of the protective efficacy of these antigenic lipids in animal models of Mtb infection.

Structural and functional definitions of Mtb glycoproteome.

We previously evidenced that protein-O-mannosylation is crucial for virulence of Mtb. Our aims are to identify and decipher the roles of the mannoproteins contributing to Mtb persistence, in vivo, and to understand the structure/function relationships of protein-O-mannosyl transferase in order to guide inhibition strategies.

Our team is involved in international projects and consortia funded by the European Union, the National Institute of Health (USA) or the Bill & Melinda Gates Foundation. It was accredited the “Équipe FRM” label by the Fondation pour la Recherche Médicale in 2018.

Main Publications

Blanc L et al. (2017) Mycobacterium tuberculosis inhibits human innate immune responses via the production of TLR2 antagonist glycolipids. Proc Natl Acad Sci U S A, Published ahead of print October 2, 2017 doi:10.1073/pnas.1707840114.

Decout A et al. (2017) Rational design of adjuvants targeting the C-type lectin Mincle. Proc Natl Acad Sci USA 114: 2675-2680

Gilleron M et al. (2016) Lysosomal lipases PLRP2 and LPLA2 process mycobacterial multiacylated lipid antigens and generate T cell stimulatory antigens. Cell Chem Biol 23: 1147-56.

Blattes E et al. (2013) Mannodendrimers prevent acute lung inflammation by inhibiting neutrophil recruitment. Proc Natl Acad Sci USA 110: 8795-800

Liu CF, Tonini L et al. (2013) Bacterial protein-O-mannosylating enzyme is crucial for virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci USA 110: 6560-5

Garcia-Alles LF et al. (2011) Structural reorganization of the antigen-binding groove of human CD1b for presentation of mycobacterial sulfoglycolipids. Proc Natl Acad Sci USA 108: 17755-60

Mannodendrimers prevent acute lung inflammation by inhibiting neutrophil recruitment. 
Lung sections of mice submitted to a LPS challenge and treated (down) or not (up; neutrophils stained in black are indicated by an arrow) with mannodendrimers.

Lipid presentation to CD1-restricted T cells

• Gilleron, M., Lepore, M., Layre, E., Cala-De Paepe, D., Mebarek, N., Shayman, J.A., Canaan, S., Mori, L., Carrière, F., Puzo, G. & De Libero, G. (2016) Lysosomal lipases PLRP2 and LPLA2 process mycobacterial multiacylated lipid antigens and generate T cell stimulatory antigens. Cell Chem Biol 23:1147-56. Pubmed
• Garcia-Alles L.F., Collmann A., Versluis C., Lindner B., Guiard J., Maveyraud L., Huc E., Im J.S., Sansano S., Brando T., Julien S., Prandi J., Gilleron M., Porcelli S.A., de la Salle H., Heck A.J., Mori L., Puzo G., Mourey L., De Libero G. (2011) Structural reorganization of the antigen-binding groove of human CD1b for presentation of mycobacterial sulfoglycolipids. Proc Natl Acad Sci USA 108:17755-60. Pubmed
• Guiard J., Collmann A., Gilleron M., Mori L., De Libero G., Prandi J. & Puzo G. (2008) Synthesis of diacylated trehalose sulfates: candidates for a tuberculosis vaccine. Angewandte Chemie 47:9734-8. Pubmed
• De la Salle H., Mariotti S., Angenieux C., Gilleron M., Garcia-Alles L.-F., Malm D., Berg T., Paoletti, S., Maître B., Mourey L., Salamero J., Cazenave J.P., Hanau D., Mori L., Puzo G. & De Libero G. (2005) Assistance of microbial glycolipid antigen processing by CD1e. Science 310:1321-1324. Pubmed
• Gilleron M., Stenger S., Mazorra Z., Wittke F., Mariotti S., Böhmer G., Prandi J., Mori L, Puzo G. & De Libero G. (2004) Diacylated sulfoglycolipids are novel mycobacterial antigens stimulating CD1-restricted T cells during infection with Mycobacterium tuberculosis. J Exp Med 199:1-12. Pubmed

Modulation of innate immune response by mycobacteria

• Bah A, Sanicas M, Nigou J, Guilhot C, Astarie-Dequeker C, & Vergne I. (2020) Lipid virulence factors of Mycobacterium tuberculosis exert a multilayered control of autophagy-related pathways in infected human macrophages. Cells. 9:666. Pubmed
• Blanc, L., Gilleron, M., Prandi, J., Song, O.-R., Jang, M.-S., Gicquel, B., Drocourt, D., Neyrolles, O., Brodin, P., Tiraby, G., Vercellone, A. & Nigou, J. (2017) Mycobacterium tuberculosis inhibits human innate immune responses via the production of TLR2 antagonist glycolipids. Proc Natl Acad Sci U S A, 114:11205-11210. Pubmed
• Decout A., Silva-Gomes S., Drocourt D., Barbe S., André I., Cueto F.J., Lioux T., Sancho D., Pérouzel E., Vercellone A., Prandi J., Gilleron M., Tiraby G. & Nigou J. (2017) Rational design of adjuvants targeting the C-type lectin Mincle. Proc Natl Acad Sci U S A 114:2675-2680. Pubmed
• Blattes, E., Vercellone, A., Eutamène, H., Turrin, C.O., Théodorou, V., Majoral, J.P., Caminade, A.M., Prandi, J., Nigou, J. & Puzo, G. (2013) Mannodendrimers prevent acute lung inflammation by inhibiting neutrophil recruitment. Proc Natl Acad Sci USA 110:8795-800. Pubmed
• Liu, C.F., Tonini, L., Malaga, W., Beau, M., Stella, A., Bouyssié, D., Jackson, M., Nigou, J., Puzo, G., Guilhot, C., Burlet-Schiltz, O. & Rivière, M. (2013) Bacterial protein-O-mannosylating enzyme is crucial for virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci USA 110:6560-5. Pubmed

Biosynthesis and structural analysis of lipids and glycoconjugates

• Tonini L, Sadet B, Stella A, Bouyssié D, Nigou J, Burlet-Schiltz O & Rivière M. (2020) Potential Plasticity of the Mannoprotein Repertoire Associated to Mycobacterium tuberculosis Virulence Unveiled by Mass Spectrometry-Based Glycoproteomics. Molecules. 25:2348. Pubmed
• Vétizou M., Pitt JM., Daillère R., Lepage P., Waldschmitt N., Flament C., Rusakiewicz S., Routy B., Roberti MP., Duong CP., Poirier-Colame V., Roux A., Becharef S., Formenti S., Golden E., Cording S., Eberl G., Schlitzer A., Ginhoux F., Mani S., Yamazaki T., Jacquelot N., Enot DP., Bérard M., Nigou J., Opolon P., Eggermont A., Woerther PL., Chachaty E., Chaput N., Robert C., Mateus C., Kroemer G., Raoult D., Boneca IG., Carbonnel F., Chamaillard M., Zitvogel L. (2015) Anticancer immunotherapy by CTLA-4 blockade relies on the gut microbiota. Science. 350:1079. Pubmed
• Larrouy-Maumus, G., Škovierová, H., Dhouib, R., Angala, S.K., Zuberogoitia, S., Pham, H., Drumond Villela, A., Mikušová, K., Noguera, A., Gilleron, M., Valentínová, L., Korduláková, J., Brennan, P.J., Puzo, G., Nigou, J. & Jackson M. (2012) A small multidrug resistance-like transporter involved in the arabinosylation of arabinogalactan and lipoarabinomannan in mycobacteria. J Biol Chem 287:39933-41.Pubmed

Reviews

• Klionsky D, ..., Vergne I, et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy 17:1-382.
• Layre E. (2020) Trafficking of Mycobacterium tuberculosis Envelope Components and Release Within Extracellular Vesicles: Host-Pathogen Interactions Beyond the Wall. Front immunol 11:1230.
• Galais M, Pradel B, Vergne I, Robert-Hebmann V, Espert L, Biard-Piechaczyk M. (2019) LAP (LC3-associated phagocytosis): phagocytosis or autophagy?Médecine/Sciences 35:635-642.
• Bah A, Vergne I. (2017) Macrophage Autophagy and Bacterial Infections. Front Immunol 8:1483. Pubmed
• Vergne I., Lafont F., Espert L.,Esclatine A., Biard-Piechaczyk M. (2017) Autophagy, ATG proteins and infectious diseases. Médecine/Sciences 33:312-318. Special Issue on "Autophagie", editorial: Patrice Codogno et Guido Kroemer. Pubmed
• Vergne, I., Gilleron, M. & Nigou, J. (2015) Manipulation of the endocytic pathway and phagocyte functions by Mycobacterium tuberculosis lipoarabinomannan. Front Cell Infect Microbiol 4, 187. Pubmed
• Layre, E., Mazurek, J. & Gilleron, M. (2015) Glycolipid Presentation by CD1. In: eLS. John Wiley & Sons, Ltd: Chichester. DOI:10.1002/9780470015902.a0020182.pub2
• Ray A., Cot M., Puzo G., Gilleron M., Nigou J. (2013) Bacterial cell wall macroamphiphiles: Pathogen-/microbe-associated molecular patterns detected by mammalian innate immune system. Biochimie 95:33-42. Pubmed

A complete list of the papers, by year

2021

• Klionsky D, ..., Vergne I, et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy 17:1-382.
• Felix J, Siebert C, Novion Ducassou J, Nigou J, Garcia PS, Fraudeau A, Huard K, Mas C, Brochier-Armanet C, Couté Y, Gutsche I & and Renesto P (2021) Structural and Functional Analysis of the Francisella Lysine Decarboxylase as a Key Actor in Oxidative Stress Resistance. Sci Rep  11:972.

2020

• Marín Franco JL, Genoula M, Corral D, Duette G, Ferreyra M, Maio M, Dolotowicz MB, Aparicio-Trejo OE, Patiño-Martínez E, Charton A, Métais A, Fuentes F, Soldan V, Moraña EJ, Palmero D, Ostrowski M, Schierloh P, Sánchez-Torres C, Hernández-Pando R, Pedraza-Chaverri J, Rombouts Y, Hudrisier D, Layre E, Vérollet C, Maridonneau-Parini I, Neyrolles O, Sasiain MDC, Lugo-Villarino G, Balboa L (2020) Host-Derived Lipids from Tuberculous Pleurisy Impair Macrophage Microbicidal-Associated Metabolic Activity. Cell Rep 33:108547.
• Källenius G, Nigou J, Cooper A & Correia-Neves M (2020) Editorial: Mycobacterial Glycolipids-Role in Immunomodulation and Targets for Vaccine Development. Front Immunol 11:603900.
• Camacho F, Moreno E, Garcia-Alles LF, Santiago GC, Gilleron M, Vasquez A, Choong YS, Reyes F, Norazmi N, Sarmiento ME & Acosta A (2020) A Direct Role for the CD1b Endogenous Spacer in the Recognition of a Mycobacterium tuberculosis Antigen by T-Cell Receptors. Front Immunol 11:566710.
• Layre E. (2020) Trafficking of Mycobacterium tuberculosis Envelope Components and Release Within Extracellular Vesicles: Host-Pathogen Interactions Beyond the Wall. Front Immunol 11:1230.
• Palčeková Z, Gilleron M, Angala SK, Belardinelli JM, McNeil M, Bermudez LE, Jackson M. (2020) Polysaccharide Succinylation Enhances the Intracellular Survival of Mycobacterium abscessus. ACS Infect Dis 6:2235-2248. 
• Le Moigne V, Roux A-L, Jobart-Malfait A, Blanc L, Chaoui K, Burlet-Schiltz O, Gaillard J-L, Canaan S, Nigou J and Herrmann J-L. (2020) A TLR2-Activating Fraction From Mycobacterium abscessus Rough Variant Demonstrates Vaccine and Diagnostic Potential. Front Cell Infect Microbiol 10:432.
• Tonini L, Sadet B, Stella A, Bouyssié D, Nigou J, Burlet-Schiltz O & Rivière M. (2020) Potential Plasticity of the Mannoprotein Repertoire Associated to Mycobacterium tuberculosis Virulence Unveiled by Mass Spectrometry-Based Glycoproteomics. Molecules 25:2348.
• Le Moigne V , Raynaud C, Moreau F, Dupont C, Nigou J, Neyrolles O, Kremer L &  Herrmann J-L. (2020) Efficacy of Bedaquiline, Alone or in Combination With Imipenem, Against Mycobacterium Abscessus in C3HeB/FeJ Mice. Antimicrob Agents Chemother 64:e00114-20.
• Dubé J-Y , McIntosh F​​​​, Zarruk JG, David S, Nigou J, & Behr MA. (2020) Synthetic Mycobacterial Molecular Patterns Partially Complete Freund's Adjuvant. Sci Rep 10:5874.
• Bah A, Sanicas M, Nigou J, Guilhot C, Astarie-Dequeker C, & Vergne I. (2020) Lipid virulence factors of Mycobacterium tuberculosis exert a multilayered control of autophagy-related pathways in infected human macrophages. Cells 9:666.

2019

• Galais M, Pradel B, Vergne I, Robert-Hebmann V, Espert L, Biard-Piechaczyk M. (2019) LAP (LC3-associated phagocytosis): phagocytosis or autophagy? Médecine/Sciences 35:635-642.
• Palčeková Z, Angala SK, Belardinelli JM, Eskandarian HA, Joe M, Brunton R, Rithner C, Jones V, Nigou J, Lowary TL, Gilleron M, McNeil M, Jackson M (2019) Disruption of the SucT acyltransferase in Mycobacterium smegmatis abrogates succinylation of cell envelope polysaccharides. J Biol Chem 294:10325-35.
• Souriant S, Balboa L, Dupont M, Pingris K, Kviatcovsky D, Cougoule C, Lastrucci C, Bah A, Gasser R, Poincloux R, Raynaud-Messina B, Al Saati T, Inwentarz S, Poggi S, Moran EJ, Gonzalez-Montaner P, Corti M, Lagane B, Vergne I, Allers C, Kaushal D, Kuroda MJ, del Carmen Sasiain M, Neyrolles O, Maridonneau-Parini I, Lugo-Villarino G, Vérollet C (2019) Tuberculosis exacerbates HIV-1 infection through IL-10/STAT3-dependent tunneling nanotube formation in macrophages. Cell Rep 26:P3586.
• Shahine A, Reinink P, Reijneveld JF, Gras S, Holzheimer M, Cheng TY, Minnaard AJ, Altman JD, Lenz S, Prandi J, Kubler-Kielb J, Moody DB, Rossjohn J & Van Rhijn I (2019) A T-cell receptor escape channel allows broad T-cell response to CD1b and membrane phospholipids. Nat Commun 10:56.
• Law C.T., Camacho F., Garcia-Alles L.F., Gilleron M., Sarmientol M., Norazmi M.N., Acosta A., Choong Y.S. (2019) Interactions of domain antibody (dAbk11) with Mycobacterium tuberculosis Ac2SGL in complex with CD1b. Tuberculosis 114:9-16

2018

• Decout, A., Silva-Gomes, S., Drocourt, D., Blattes, E., Rivière, M., Prandi, J., Larrouy-Maumus, G., Caminade, A.-M., Hamasur, B., Källenius, G., Kaur, D., Dobos, K.M., Lucas, M., Sutcliffe, I.C., Besra, G.S., Appelmelk, B.J., Gilleron, M., Jackson, M., Vercellone, A., Tiraby, G. & Nigou J. (2018) Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2. Sci Rep 8, 16840.
• Nigou, J., Prandi, J., Blattes, E., Silva-Gomes, S., Caminade, A.-M., Vercellone, A. & Puzo, G. (2018) Rational design of mannodendrimers targeting human C-type lectin receptors to prevent lung inflammation. In Phosphorus dendrimers in biology and medicine: syntheses, characterization, and properties (Caminade, A.-M., Majoral, J.-P. & Turrin, C.-O., eds), pp 265-85, Pan Stanford Publishing Pte. Ltd., Singapore.
• James C.A., Yu K.K.Q., Gilleron M., Prandi J., Yedulla V.R., Moleda Z.Z., Diamanti E., Khan M., Aggarwal V.K., Reijneveld J.F., Reinink P., Lenz S., Emerson R.O., Scriba T.J., Souter M.N.T., Godfrey D.I., Pellicci D.G., Moody D.B., Minnaard A.J., Seshadri C., Van Rhijn I. (2018) CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis. Cell Chem Biol 2018 Jan 29. pii: S2451-9456(18)30006-0. doi: 10.1016/j.chembiol.2018.01.006.
• Tersa M., Raich L., Albesa-Jové D., Trastoy B., Prandi J., Gilleron M., Rovira C., Guerin M.E. (2018) The molecular mechanism of substrate recognition and catalysis of the membrane acyltransferase PatA from Mycobacteria. ACS Chem Biol 13:131-140
• Oldenburg R., Mayau V., Prandi J., Arbues A., Astarie-Dequeker C., Guilhot C., Werts C., Winter N., Demangel C. (2018) Mycobacterial Phenolic Glycolipids Selectively Disable TRIF-Dependent TLR4 Signaling in Macrophages. Front Immunol 2018 Jan 19;9:2. doi: 10.3389/fimmu.2018.00002. eCollection 2018.

2017

• Kaufmann SHE, Dockrell HM, Drager N, Ho MM, McShane H, Neyrolles O, Ottenhoff THM, Patel B, Roordink D, Spertini F, Stenger S, Thole J, Verreck FAW, Williams A; TBVAC2020 Consortium. (2017) TBVAC2020: Advancing Tuberculosis Vaccines from Discovery to Clinical Development. Front Immunol 8:1203.
• Alonso H, Parra J, Malaga W, Payros D, Liu CF, Berrone C, Robert C, Meunier E, Burlet-Schiltz O, Rivière M, Guilhot C. (2017) Protein O-mannosylation deficiency increases LprG-associated lipoarabinomannan release by Mycobacterium tuberculosis and enhances the TLR2-associated inflammatory response. Sci Rep 7:7913
• Bah A, Vergne I. (2017) Macrophage Autophagy and Bacterial Infections. Front Immunol 8:1483.
• Blanc, L., Gilleron, M., Prandi, J., Song, O.-R., Jang, M.-S., Gicquel, B., Drocourt, D., Neyrolles, O., Brodin, P., Tiraby, G., Vercellone, A. & Nigou, J. (2017) Mycobacterium tuberculosis inhibits human innate immune responses via the production of TLR2 antagonist glycolipids. Proc Natl Acad Sci U S A 114:11205-11210
• Gharun K., Senges J., Seidl M., Lösslein A., Kolter J., Lohrmann F., Fliegauf M., Elgizouli M., Vavra M., Schachtrup K., Illert L., Gilleron M., Kirschning C., Triantafyllopoulou A. & Henneke P. (2017) Mycobacteria exploit nitric oxide-induced transformation of macrophages into permissive giant cells. EMBO Rep 18:2144-2159
• Robert, C.B., Thomson, M., Vercellone, A., Gardner, F., Ernst, R.K., Larrouy-Maumus, G. & Nigou, J. (2017) Mass spectrometry analysis of intact Francisella bacteria identifies lipid A structure remodeling in response to acidic pH stress. Biochimie 141, 16-20.
• Nigou, J., Touqui, L. & Record, M. (2017) Microbe and host lipids. Biochimie 141, 1-2.
• Vergne I., Lafont F., Espert L.,Esclatine A., Biard-Piechaczyk M. (2017) Autophagy, ATG proteins and infectious diseases.Médecine/Sciences 33:312-318. Special Issue on "Autophagie", editorial: Patrice Codogno et Guido Kroemer.
• Decout A., Silva-Gomes S., Drocourt D., Barbe S., André I., Cueto F.J., Lioux T., Sancho D., Pérouzel E., Vercellone A., Prandi J., Gilleron M., Tiraby G. & Nigou J. (2017) Rational design of adjuvants targeting the C-type lectin Mincle. Proc Natl Acad Sci U S A 114:2675-2680.
• Larrouy-Maumus, G., Layre, E., Clark, S., Prandi, J., Rayner, E., Lepore, M., De Libero, G., Williams, A., Puzo, G. & Gilleron, M. (2017) Protective efficacy of a lipid antigen vaccine in a guinea pig model of tuberculosis. Vaccine 35:1395-1402.
• Parra, J., Marcoux, J., Poncin, I., Canaan, S., Hermann, J.-L., Nigou, J., Burlet-Schiltz, O. & Rivière, M. (2017) Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm. Sci Rep 7:43682.
• Angala, S.K., McNeil, M.R., Shi, L., Joe, M., Pham, H., Zuberogoitia, S., Nigou, J., Boot, C.M., Lowary, T.L., Gilleron, M. & Jackson, M. (2017) Biosynthesis of the methylthioxylose capping motif of lipoarabinomannan in Mycobacterium tuberculosis. ACS Chem Biol 12:682-691.
• Troegeler, A., Mercier, I., Cougoule, C., Colom, A., Duval, C., Vu Manh, T.-P., Capilla, F., Poincloux, R., Pingris, K., Nigou, J., Rademann, J., Dalod, M., Verreck, F.A.W., Al Saati, T., Lugo-Villarino, G., Lepenies, B., Hudrisier, D. & Neyrolles, O. (2017) C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci USA 114:E540-E549.
• Jankute, M., Alderwick, L.J., Noack, S., Veerapen, N., Nigou, J. & Besra, G.S. (2017) Disruption of Mycobacterial AftB Results in Complete Loss of Terminal β(1 → 2) Arabinofuranose Residues of Lipoarabinomannan. ACS Chem Biol 12:183-190.
• Deboosère, N., Iantomasi, R., Queval, C.J., Song, O.R., Deloison, G., Jouny, S., Debrie, A.S., Chamaillard, M., Nigou, J., Cohen-Gonsaud, M., Locht, C., Brodin, P. & Veyron-Churlet, R. (2017) LppM impact on the colonization of macrophages by Mycobacterium tuberculosis. Cell Microbiol 19:e12619.

2016

• Roux, A.L., Viljoen, A., Bah, A., Simeone, R., Bernut, A., Laencina, L., Deramaudt, T., Rottman, M., Gaillard, J.L., Majlessi, L., Brosch, R., Girard-Misguich, F., Vergne, I., de Chastellier, C., Kremer, L. & Herrmann, J.L. (2016) The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages. Open Biol 6:160185.
• Gilleron, M., Lepore, M., Layre, E., Cala-De Paepe, D., Mebarek, N., Shayman, J.A., Canaan, S., Mori, L., Carrière, F., Puzo, G. & De Libero, G. (2016) Lysosomal lipases PLRP2 and LPLA2 process mycobacterial multiacylated lipid antigens and generate T cell stimulatory antigens. Cell Chem Biol 23:1147-56.
• Arbues, A., Malaga, W., Constant, P., Guilhot, C., Prandi, J. & Astarie-Dequeker, C. (2016) Trisaccharides of Phenolic Glycolipids Confer Advantages to Pathogenic Mycobacteria through Manipulation of Host-Cell Pattern-Recognition Receptors. ACS Chem Biol 11:2865-75.
• Barthe, P., Veyron-Churlet, R., de Visch, A., Gilleron, M., Saliou, J.-M., Tomavo, S., Nigou, J., Brodin, P. & Cohen-Gonsaud, M. (2016) Mycobacterium tuberculosis LppM displays an original structure and domain composition linked to a dual localisation. Structure 24:1788-1794.
• Bah, A., Lacarriere, C. & Vergne, I. (2016) Autophagy-related proteins target ubiquitin-free mycobacterial compartment to promote killing in macrophages. Front Cell Infect Microbiol 6:53.
• Kaufmann, E., Spohr, C., Battenfeld, De Paepe, D., Holzhauser, Balks, E., Homolka, S., Reiling, N., Gilleron, M., Bastian, M. (2016) BCG-vaccination induces robust CD4+ T-cell responses to Mycobacterium tuberculosis complex-specific lipopeptides in guinea pigs. J Immunol 196(6):2723-32.
• Camacho F., Sarmiento M.E., Reyes F., Kim L., Huggett J., Lepore M., Otero O., Gilleron M., Puzo G., Norazmi M. N., Rook G., Mori L., De Libero G. & Acosta A. (2016) Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL. Int. J. Mycobacteriol. 5:120-7.

2015

• Vétizou, M., Pitt, J.M., Daillère, R., Lepage, P., Waldschmitt, N., Flament, C., Rusakiewicz, S., Routy, B., Roberti, M.P., Duong, C.P.M., Poirier-Colame, V., Roux, A., Becharef, S., Formenti, S., Golden, E., Cording, S., Eberl, G., Schlitzer, A., Ginhoux, F., Mani, S., Yamazaki, T., Jacquelot, N., Enot, D.P., Bérard, M., Nigou, J., Opolon, P., Eggermont, A., Woerther, P.L., Chachaty, E., Chaput, N., Robert, C., Mateus, C., Kroemer, G., Raoult, D., Gomperts Boneca, I., Carbonnel, F., Chamaillard, M. & Zitvogel, L. (2015) Anticancer immunotherapy by CTLA4 blockade relies on the gut microbiota. Science 350(6264):1079-84.
• Seshadri C., Lin L., Scriba T.J., Peterson G., Freidrich D., Frahm N., DeRosa S.C., Moody D.B., Prandi J., Gilleron M., Mahomed H., Jiang W., Finak G., Hanekom W.A., Gottardo R., McElrath M.J., Hawn T.R. (2015) T Cell Responses against Mycobacterial Lipids and Proteins Are Poorly Correlated in South African Adolescents. J Immunol 195(10):4595-603.
• Prakhar P., Holla S., Ghorpade D.S., Gilleron M., Puzo G., Udupa V., Balaji K.N. (2015) Ac2PIM-responsive miR-150 and miR-143 Target Receptor-interacting Protein Kinase 2 and Transforming Growth Factor Beta-activated Kinase 1 to Suppress NOD2-induced Immunomodulators. J Biol Chem, 290(44):26576-86.
• Larrouy-Maumus, G., Gilleron, M., Skovierová, H., Zuberogoitia, S., Brennan, P.J., Puzo, G., Jackson, M. & Nigou, J. (2015) A glycomic approach reveals a new mycobacterial polysaccharide. Glycobiology 25(11):1163-71.
• Layre, E., Mazurek, J. & Gilleron, M. (2015) Glycolipid Presentation by CD1. In: eLS. John Wiley & Sons, Ltd: Chichester. DOI:10.1002/9780470015902.a0020182.pub2
• Troegeler, A., Lugo-Villarino, G., Hansen, S, Rasolofo, V, Henriksen, M.L., Mori, K., Ohtani, K., Duval., C, Mercier., I, Bénard., A, Nigou, J., Hudrisier, D., Wakamiya, N. & Neyrolles, O. (2015) Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis. PLoS One 10(7), e0132692.
• Wheat, W.H., Dhouib, R., Angala, S.K., Larrouy-Maumus, G., Dobos, K., Nigou, J., Spencer, J.S. & Jackson, M. (2015) The Presence of a Galactosamine Substituent on the Arabinogalactan of Mycobacterium tuberculosis Abrogates Full Maturation of Human Peripheral Blood Monocyte-Derived Dendritic cells and Increases Secretion of IL-10. Tuberculosis 95(4), 476-89.
• Espert, L., Beaumelle, B. & Vergne, I. (2015) Autophagy in Mycobacterium tuberculosis and HIV infections. Front Cell Infect Microbiol 5:49.
• Wang, J., Gasc, F. & Prandi, J. (2015) Samarium Diiodide Mediated Coupling of 2-Pyridylsulfonyl Furanosides with Aldehydes and Ketones: A General Synthesis of C-Furanosides. Eur J Org Chem 12, 2691–2697.
• Vergne, I., Gilleron, M. & Nigou, J. (2015) Manipulation of the endocytic pathway and phagocyte functions by Mycobacterium tuberculosis lipoarabinomannan. Front Cell Infect Microbiol 4, 187.
• Lanéelle, M.-A., Nigou, J. & Daffé, M. (2015) Lipid and lipoarabinomannan purification and characterization. Methods Mol Biol 1285, 77-103.
• Blattes, E., Vercellone, A., Gomes-Silva, S., Prandi, J. & Nigou, J. (2015) Molecular bases of Mycobacterium tuberculosis recognition by the C-type lectin DC-SIGN: from the modulation of innate immune response to the design of innovative anti-inflammatory drugs. In Carbohydrates in Drug Design and Discovery (Jimenez-Barbero, J., Javier Canada, F. & Martin-Santamaria, S., eds), pp 64-88, The Royal Society of Chemistry, Cambridge, UK.
• Larrouy-Maumus, G. & Puzo, G. (2015) Mycobacterial envelope lipids fingerprint from direct MALDI-TOF MS analysis of intact bacilli. Tuberculosis 95:75-85.

2014

• Gomes-Silva, S., Decout, A. & Nigou, J. (2014) Pathogen-Associated Molecular Patterns (PAMPs). In Encyclopedia of Inflammatory Diseases (Parnham BSc, PhD, CBiol MSB, FBPharmacolS M., Ed). SpringerReference (www.springerreference.com). Springer Basel. DOI 10.1007/978-3-0348-0620-6_35-1.

2013

• Blanc, L., Castanier, R., Mishra, A.K., Ray, A., Besra, G.S., Sutcliffe, I., Vercellone, A., & Nigou, J. (2013) Gram-positive bacterial lipoglycans based on a glycosylated diacylglycerol lipid anchor are microbe-associated molecular patterns recognized by TLR2. PLoS One 8:e81593.
• Gau, B., Lemétais, A., Lepore, M., Garcia-Alles, L.-F., Bourdreux, Y., Mori, L., Gilleron, M., De Libero, G., Puzo, G., Beau, J.-M. & Prandi, J. (2013) Simplified Deoxypropionate Acyl Chains for Mycobacterium tuberculosis Sulfoglycolipid Analogues: Chain Length is Essential for High Antigenicity. ChemBioChem 14:2413-7.
• Liu, C.-F., Drocourt, D., Puzo, G., Wang, J.-Y. & Rivière, M. (2013) Innate immune response of alveolar macrophage to house dust mite allergen is mediated through TLR2/-4 co-activation. PLoS One 8:e75983.
• Blattes, E., Vercellone, A., Eutamène, H., Turrin, C.O., Théodorou, V., Majoral, J.P., Caminade, A.M., Prandi, J., Nigou, J. & Puzo, G. (2013) Mannodendrimers prevent acute lung inflammation by inhibiting neutrophil recruitment. Proc Natl Acad Sci USA 110:8795-800.
• Liu, C.F., Tonini, L., Malaga, W., Beau, M., Stella, A., Bouyssié, D., Jackson, M., Nigou, J., Puzo, G., Guilhot, C., Burlet-Schiltz, O. & Rivière, M. (2013) Bacterial protein-O-mannosylating enzyme is crucial for virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci USA 110: 6560-5.
• Gouzy, A., Nigou, J., Gilleron, M., Neyrolles, O., Tailleux, L. & Gordon, S.V. (2013) Tuberculosis 2012: Biology, pathogenesis and intervention strategies-an update from the City of Light. Res Microbiol 164: 270-80.
• Afonso-Barroso, A., Clark, S.O., Williams, A., Rosa, G.T., Nóbrega, C., Silva-Gomes, S., Vale-Costa, S., Ummels, R., Stoker, N., Movahedzadeh, F., van der Ley, P., Sloots, A., Cot, M., Appelmelk, B.J., Puzo, G., Nigou, J., Geurtsen, J. & Appelberg, R. (2013) Lipoarabinomannan mannose caps do not affect mycobacterial virulence or the induction of protective immunity in experimental animal models of infection and have minimal impact on in vitro inflammatory responses. Cell Microbiol 15: 660-74.
• Geerdink, D., ter Horst, B., Lepore, M. Mori, L., Puzo, G., Hirsch, A., Gilleron, M., de Libero, G.& Minnaard, A. (2013) Total synthesis, stereochemical elucidation and biological evaluation of Ac2SGL; a 1,3-methyl branched sulfoglycolipid from Mycobacterium tuberculosis. Chem Sci 4: 709–716.
• Ray A., Cot M., Puzo G., Gilleron M., Nigou J. (2013) Bacterial cell wall macroamphiphiles: Pathogen-/microbe-associated molecular patterns detected by mammalian innate immune system. Biochimie 95: 33-42.

2012

• Mishra, A.K., Alves, J.E., Krumbach, K., Nigou, J., Castro, A.G., Geurtsen, J., Eggeling, L., Saraiva, M. & Besra G.S. (2012) Differential arabinan capping of lipoarabinomannan modulates innate immune responses and impacts T helper cell differentiation. J Biol Chem 287:44173-83.
• Larrouy-Maumus, G., Škovierová, H., Dhouib, R., Angala, S.K., Zuberogoitia, S., Pham, H., Drumond Villela, A., Mikušová, K., Noguera, A., Gilleron, M., Valentínová, L., Korduláková, J., Brennan, P.J., Puzo, G., Nigou, J. & Jackson M. (2012) A small multidrug resistance-like transporter involved in the arabinosylation of arabinogalactan and lipoarabinomannan in mycobacteria. J Biol Chem 287: 39933-41.
• Cala-De Paepe D., Layre E., Giacometti G., Garcia-Alles L.F., Mori L., Hanau D., De Libero G., de la Salle H., Puzo G., Gilleron M. (2012) Deciphering the role of CD1e in mycobacterial phosphatidyl-myo-inositol-mannosides (PIM) processing for presentation by CD1b to T lymphocytes. J Biol Chem 287:31494-502.
• Prandi, J., A Convenient Synthesis of Glucose Monomycolate. Carbohydr Res 2012, 347:151–154.

2011

• Krishna, S., Ray, A., Dubey, S.K., et al., Lipoglycans contribute to innate immune detection of mycobacteria. PLoS One, 2011, 6(12):e28476.
• Cot, M., et al., Lipoteichoic acid in Streptomyces hygroscopicus: Structural model and immunomodulatory activities. PLoS One 2011, 6(10):e26316.
• Garcia-Alles, L.F., et al., Structural reorganization of the antigen-binding groove of human CD1b for presentation of mycobacterial sulfoglycolipids. Proc Natl Acad Sci USA, 2011, 108(43):17755-60.
• Facciotti, F., Fine tuning by human CD1e of lipid-specific immune responses. Proc Natl Acad Sci USA 2011, 108(34):14228-33.
• Garcia-Alles, L.F., et al., Crystal structure of human CD1e reveals a groove suited for lipid-exchange processes. Proc Natl Acad Sci USA 2011, 108(32):13230-5.
• Layre, E., et al., Deciphering sulfoglycolipids of Mycobacterium tuberculosis. J Lipid Res 2011. 52(6): p.1098-1110.
• Baud C., et al., Membrane-associated DegP in Bordetella chaperones a repeat-rich secretory protein. Mol Microbiol 2011, 80(6):1625-36.
• Mishra, A.K., et al., Lipoarabinomannan biosynthesis in Corynebacterineae: the interplay of two α(1→2)-mannopyranosyltransferases MptC and MptD in mannan branching. Mol Microbiol 2011. 80(5): p.1241-1259.
• Roux, A.L., et al., Overexpression of proinflammatory TLR-2-signalling lipoproteins in hypervirulent mycobacterial variants. Cell Microbiol 2011, 13(5): p.692–704.

2010

• Birch, H.L., et al., A truncated lipoglycan from mycobacteria with altered immunological properties. Proc Natl Acad Sci U S A, 2010. 107(6): p. 2634-9.
• Astarie-Decqueker, et al., The role of mycobacterial lipids in host pathogenesis. Drug Discov Today (Disease Mechanisms), 2010. 7(1): p.e33-41.
• Puzo, G., Lipidomique. Médecine Clinique, 2010. 46: p.30-35.
• Rivière, M., Les défis de la glycomique clinique. Médecine Clinique, 2010. 47: p.22-26.
• Brodin, P., et al., High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling. PLoS Pathog, 2010. 6(9): e1001100.
• Driessen, N.N., et al., Mycobacterium marinum MMAR_2380, a predicted transmembrane acyltransferase, is essential for the presence of the mannose cap on lipoarabinomannan. Microbiology, 2010. 156(Pt 11): p. 3492-502.
• Jang, J., et al., Functional characterization of the Mycobacterium tuberculosis serine/threonine kinase PknJ. Microbiology, 2010. 156(Pt 6): p. 1619-31.
• Liu, C.F., et al., Surfactant protein D inhibits mite-induced alveolar macrophage and dendritic cell activations through TLR signalling and DC-SIGN expression. Clin Exp Allergy, 2010. 40(1): p. 111-22.
• Skovierova, H., et al., Biosynthetic origin of the galactosamine substituent of Arabinogalactan in Mycobacterium tuberculosis. J Biol Chem, 2010. 285(53): p. 41348-55.

Download the complete list of publications from 2016 to 1989

Patents

Dendrimères à terminaison saccharide à visée anti-inflammatoire. Blattes, E., Puzo, G., Prandi, J., Nigou, J., Vercellone, A., Majoral, J.P., Turrin, C.O., Caminade A.M. 

Patent n° 10 56902 (FR, 31/08/2010), 11764827.9 (EU, 31/08/2011), 13/819872 (USA, 31/08/2011).

Pharmaceutical compositions comprising actinomycete glycerol acyl derivatives antigens, their process of extraction, and their use against tuberculosis. Puzo, G., Layre, E., Gilleron, M., Prandi, J., Stenger, S., De Libero, G. 

EP20070291249, deposited 12/10/2007, extended 13/10/2008.

Composition pour la prévention et/ou le traitement des maladies associées à la surexpression du TNF et/ou de l’IL12. Quesniaux, V., Gilleron, M., Puzo, G., Nigou, J. 

FR20060010136, deposited 20/11/2006, extended 20/11/2007.

Sulfoglycolipid antigens, their process of preparation, and their use against tuberculosis. Puzo, G., Prandi, J., Gilleron, M., De Libero, G., Guiard, J., Mori, L., Paoletti, S. EP20070290097, deposited 24/01/2007, extended 13/10/2008.

Sulfoglycolipid antigens, their extraction from Mycobacterium tuberculosis, and their use against tuberculosis. Puzo, G., Gilleron, M., Stenger, S., De Libero, G. EP20030290965, deposited 18/04/2003.

Collaborations

International

• M. Bastian, Paul Ehrlich-Institut, Langen, Germany
• M. Behr, McGill University Health Centre Research Institute, Montréal, Canada
• G.S. Besra, University of Birmingham, UK
• M. Guerin, CICbioGUNE, Derio, Spain
• M. Jackson and P.J. Brennan, Colorado State University, Fort Collins, USA
• G. De Libero, University Hospital Basel, Bâle, Switzerland
• R. Malley, Boston Children's Hospital, Boston, USA
• K. Mikusova, Comenius University, Bratislava, Slovak Republic
• D.B. Moody, Brigham and Women’s Hospital, Boston, USA
• A. Rawkins, Health Protection Agency, Salisbury, UK
• S. Stenger, Institut für Med. Mikrobiologie und Hygiene, Ulm, Germany
• C. Seshadri, University of Washington, Seattle, USA

National

• I. André and S. Barbe, INSA, Toulouse
• Y. Bourne, CNRS, Marseille
• P. Brodin, Institut Pasteur, Lille
• O. Burlet-Shiltz, IPBS
• A.M. Caminade, CNRS, Toulouse
• S. Canaan, CNRS, Marseille
• F. Carrière, CNRS, Marseille
• M. Chavent, IPBS
• C. Guilhot, IPBS
• J.-L. Herrmann, Université de Versailles Saint-Quentin en Yvelines
• O. Neyrolles, IPBS
• A. Peixoto, IPBS

Industry

• Indus Biotech, Pune, India
• Invivogen, Toulouse, France
• Siamed’xpress, Marseille, France

Funding

Our team is labelled by the Fondation pour la Recherche Médicale (2018-2021). 

Our team  is or has been supported in the five past years by grants or fellowships from :

International

• Bill & Melinda Gates Foundation (BMGF)
• Centre Franco-Indien pour la Promotion de la Recherche Avancée (CEFIPRA/IFCPAR)
• Chinese Scholarship Council (CSC)   
• European Union (EU)
• National Institute of Health (NIH, USA)
• Swedish Research Council (Vetenskapsrådet)

National

• Agence Nationale de la Recherche (ANR)
• Association Nationale de la Recherche et de la Technologie (ANRT)
• Centre National de la Recherche Scientifique (CNRS)
• Direction Générale de l’Armement (DGA)
• Région Occitanie (web)
• Toulouse Tech Transfer (TTT)
• Université Paul Sabatier de Toulouse (UPS)

Charities

• Fondation pour la Recherche Médicale (FRM)
• Vaincre la Mucoviscidose (VLM)
• Fondation Roland Garrigou (Fonroga) 

Industry

• Institut Mérieux (web)
• Invivogen (web)
• Indus Biotech (web)
• MSDAvenir (web)