Mass spectrometry workflow for identification of cleavages sites. Using highly sensitive mass spectrometry, we identified the N-terminal peptides characteristic of the cleavages generated on IL-33 by a whole panel of allergens. These cleavage events induce activation of the cytokine, finally triggering asthma.
Selected publications
- Lefrancais et al. IL-33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G. Proc Natl Acad Sci U S A 2012. https://doi.org/10.1073/pnas.1115884109
- Cayrol et al. Environmental allergens induce allergic inflammation through proteolytic maturation of IL-33. Nat. Immunol. 2018. https://doi.org/10.1038/s41590-018-0067-5