We develop single molecule approaches to characterize the dynamics of macromolecular assemblies of nucleic acids with basic science and application-driven objectives.
Biophysics
The first objective comprises the physical and biochemical principles of the organization of the bacterial nucleoid, in relation with genome repair functions in particular, and the role of repeated sequences in molecular mechanisms altered by cancer.
Biosensing
The second objective evaluates the combination of our high-throughput Tethered Particle Motion technique with recognition elements as a sensor for water micropollutants, biomarkers and screening of therapeutics ligands.
Current Fundings
ANR-20-CE04-0010
University Paul Sabatier 2021-2023
CNRS MITI 2021
Personnel involved
Serge Mazères, Laurence Salomé, Catherine Tardin
Collaborators
Vincent Ecochard IPBS, Toulouse
Dennis Gomez IPBS, Toulouse
Nicolas Destainville and Manoel Manghi, LPT, Toulouse
Philippe Rousseau and François Cornet, CBI-LMGM, Toulouse
Raphael Mourad, CBI-MCD, Toulouse
Jean-Marc Escudier, Cécile Dehoux-Baudouin, Béatrice Gerland, Stéphanie Ballereau, SPCMIB, Toulouse
Marc Joyeux, LiPhy, Grenoble
Aurélien Olichon, DéTROI, La Réunion
Selected publications
- Serres S., Tardin C. and Salomé L. (2020) Single-molecule sensing of DNA intercalating drugs in water Anal. Chem. 92(12):8151-8158 doi: 10.1021/acs.analchem.0c00184.
- Soukarié D., Ecochard V. and Salomé L. (2020) DNA nanobiosensors for the monitoring of water quality Int J Hyg Environ Health 226:113485 doi: 10.1016/j.ijheh.2020.113485
- Allemand J.-F., Tardin C. and Salomé L. (2019) Parallelized DNA tethered bead measurements to scrutinize DNA mechanical structure Methods 169, 46-56 doi.org/10.1016/j.ymeth.2019.07.020
- Guilbaud S., Salomé L., Destainville N., Manghi M., Tardin C. (2019) Dependence of DNA persistence length on ionic strength and ion type. Phys. Rev. Lett. 122, 028102
- Brunet A., Salomé L., Rousseau P., Destainville N., Manghi M. and Tardin C. (2018) How does temperature impact the conformation of single DNA molecules below melting temperature? Nucleic Acids Res. 46(4):2074-2081 doi: 10.1093/nar/gkx1285
- Fournes F., Crozat E., Pages C., Tardin C., Salomé L., Cornet F. and Rousseau P. (2016) FtsK translocation permits discrimination between an endogenous and an imported Xer/dif recombination complex PNAS 113 (28), 7882–7887
- Brunet A., Tardin C., Salomé L., Rousseau P., Destainville N. and Manghi M. (2015) Dependence of DNA persistence length on ionic strength of solutions with monovalent and divalent salts: a joint theory-experiment study. Macromolecules 48 (11), 3641–3652 doi: 10.1021/acs.macromol.5b00735
- Brunet A, Chevalier S, Destainville N, Manghi M, Rousseau P, Salhi M, Salomé L, Tardin C. (2015) Probing a label-free local bend in DNA by single molecule tethered particle motion Nucleic Acids Res. 43(11):e72 doi: 10.1093/nar/gkv201