- Cell culture : murine adipocyte (3T3F442A), normal and tumoral breast and prostate cells lines, melanoma cell lines
- Isolation of Primary adipocytes from mouse and human adipose tissue (collaboration Philippe Valet)
- Coculture of adipocytes with tumor cells 2D and 3D models including spheroids
- Tumor cell xenograft in lean and obese mice (collaboration Philippe Valet)
- Characterization of adipocytes phenotype: adipose cell markers (Q-PCR, Western Blot), Red oil/Bodipy labeling, measure of triglycerides content
- Molecular Biology: real time quantitative PCR, si/shRNA…
- Biochemistry: Western blot, Immuno-precipitation, Elisa…
- Immunofluorescence /Confocal Imaging /Time lapse
- Wound Healing assay, Migration and Invasion Assays using Transwells
