Reinforcement of the PICT platform's Biophysics facility with the integration of spectral shift and nanoDSF technologies

13 June 2023 9h30-13h00

The PICT facility and NanoTemper Technologies are organizing a symposium on biophysical characterization on June 13, 2023 at PBS, starting at 9:30 am.
The symposium is free of charge. However, for organizational reasons, registration is mandatory. The deadline for registration is June 12.

Programme

09h30 Welcome
09h45 Introduction Nicolas Becker, Technical Sales Specialist, NanoTemper Technologies

09h55 PICT platform presentation Dr. Virginie Nahoum, IPBS PICT platform manager, CNRS Toulouse

10h15 Prometheus Panta: the new gold standard for protein characterization Nicolas Becker, Technical Sales Specialist , NanoTemper Technologies

10h30 Application examples of nanoDSF for the study of antibodies, protein/protein complexes and protein/nucleic acids complexes (CRIPR-CAS9) Karine Bernardeau, P2R, INSERM Nantes

10h50 Break
11h15 Monolith and Dianthus: MST and spectral shift for affinities measurements Dr. Pierre Soule, Application specialist, NanoTemper Technologies

11h30 Complementarity of biophysical approaches for the identification of ligands (peptides, lipids, DNA) and the sample preparation for structural studies (crystallography, NMR, cryo-EM) Dr. Valérie Guillet, IPBS, CNRS Toulouse

11h50 How do crystallography and spectral shift help to decipher a dual activity enzyme mechanism Dr. Virginie Nahoum, IPBS, CNRS Toulouse

12h10 Determination of protein/nucleic acid interaction thermodynamic parameters by microcalorimetry (ITC) Dr. Virginie Gervais, I2BC, CNRS Paris-Saclay

12h30 Conclusion

Reinforcement of the PICT platform's Biophysics facility with the integration of spectral shift and nanoDSF technologies