Cellular Biophysics

We develop a multidisciplinary approach combining cell biology and biophysics to determine the mechanisms of membrane perturbations induced by transmembrane potential modifications, i.e. by the electroporation or electropermeabilization technique.These studies bring new approaches and guidelines for safe and efficient delivery of therapeutic molecules into cells and tissues since delivery requires targeting molecules towards specific cells.

In that context, and based on our previous works, the main objectives we pursued are:

•Elucidation of the mechanisms of electrotransfer: our works give evidence of the differences between the transfer of small molecules (bleomycin, cis-platinium), small oligonucleotides (siRNA, LNA), proteins and plasmid DNA and between the mechanisms involved in vitro on cells in culture (2-dimensional and 3-dimensional models) and in vivo on tissus (murine model).

•Development of new tools for delivery, targeting and diagnostic: our results have led us to define new types of electrical pulse generators and electrodes (adapted for study in vitro and in vivo models) and to adapt imaging systems (dorsal window chamber, fluorescence micro and macroscopies). In addition, we are developing other non-viral drug delivery approach based on lectin and we are using DNA aptamers that bind specifically to cancer cells. This approach has interests for Targeting of tumours (drug delivery) and Diagnostics (imaging of solid tumors).

We work in collaboration with laboratories worldwide in the context of several international networks (International Bioelectrics Consortium, COST Action TD1104: European network for development of electroporation-based technologies and treatments and LIA-EBAM: International Associated Laboratory on Pulsed Electric Fields Applications in Biology and Medicine). 

Main Publications

•    Ladurantie C et al 2019. A protein nanocontainer targeting epithelial cancers: rational engineering, biochemical characterization, drug loading and cell delivery. Nanoscale    
•    Markelc B et al 2018. Increased permeability of blood vessels after reversible electroporation is facilitated by alterations in endothelial cell-to-cell junctions. J Control Release. 276:30-41.
•    Pillet F et al 2017. Importance of endogenous extracellular matrix in biomechanical properties of human skin model. Biofabrication 9:025017
•    Pillet F et al 2016. Cell wall as a target for bacteria inactivation by pulsed electric fields. Sci  Rep. 6:19778.
•    Rosazza C et al 2016. Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro. Mol Ther Nucleic Acids. 5:1-11. 
Paganin-Gioanni A et al. (2011). Direct visualization at the single-cell level of siRNA electrotransfer into cancer cells. Proc Natl Acad Sci USA 108: 10443-7

Biological systems of increasing complexities to unravel the mechanisms of molecules delivery into cells by electrotransfer, DNA aptamer and lectin.

Team Publications

2019

• Ladurantie. C.#, Coustets, M.#, Czaplicki, G., P. Demange, S. Mazeres, S. Dauvillier, J. Teissié, M-P. Rols, A. Milon, V. Ecochard, G. Gross and L.Paquereau*. (2019) A protein nanocontainer targeting epithelial cancers: rational engineering, biochemical characterization, drug loading and cell delivery. Nanoscale 14;11(7):3248-3260.
• Chauvin J, Gibot L, Griseti E, Golzio M, Rols MP, Merbahi N, Vicendo P. Elucidation of in vitro cellular steps induced by antitumor treatment with plasma-activated medium.(2019) Sci Rep. 19;9(1):4866.
• Bocé M, Tassé M, Mallet-Ladeira S, Pillet F, Da Silva C, Vicendo P, Lacroix PG, Malfant I, Rols MP. (2019) Effect of trans(NO, OH)-[RuFT(Cl)(OH)NO](PF6) ruthenium nitrosyl complex on methicillin-resistant Staphylococcus epidermidis. Sci Rep. 19;9(1):4867.
• Pillet F, Dague E, Pečar Ilić J, Ružić I, Rols MP, Ivošević DeNardis N.(2019) Changes in nanomechanical properties and adhesion dynamics of algal cells during their growth. Bioelectrochemistry. 127:154-162.
• Pasquet L, Chabot S, Bellard E, Rols MP, Teissie J, Golzio M. 2019. Noninvasive Gene Electrotransfer in Skin. Hum Gene Ther Methods. 30(1):17-22.
• Phonesouk E, Lechevallier S, Ferrand A, Rols MP, Bezombes C, Verelst M, Golzio M. (2019) Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications. Materials (Basel). 7;12(1).

2018

• Pasquet, L., S. Chabot, E. Bellard, B. Markelc, M. P. Rols, J. P. Reynes, G. Tiraby, F. Couillaud, J. Teissie*, and M. Golzio*. (2018) Safe and efficient novel approach for non-invasive gene electrotransfer to skin. Sci  Rep 8:16833.
• Markelc, B., E. Bellard, G. Sersa, T. Jesenko, S. Pelofy, J. Teissie, R. Frangez, M. P. Rols, M. Cemazar*, and M. Golzio*. (2018) Increased permeability of blood vessels after reversible electroporation is facilitated by alterations in endothelial cell-to-cell junctions. J Control Release 276:30-41.
• De Robertis, M., L. Pasquet, L. Loiacono, E. Bellard, L. Messina, S. Vaccaro, R. Di Pasquale, V. M. Fazio, M. P. Rols, J. Teissie*, M. Golzio*, and E. Signori*. (2018) In Vivo Evaluation of a New Recombinant Hyaluronidase to Improve Gene Electro-Transfer Protocols for DNA-Based Drug Delivery against Cancer. Cancers (Basel). 10.
• Ric, A., V. Ecochard, J. S. Iacovoni, A. Boutonnet, F. Ginot, V. Ong-Meang, V. Poinsot, L. Paquereau, and F. Couderc. (2018) G-quadruplex aptamer selection using capillary electrophoresis-LED-induced fluorescence and Illumina sequencing. Anal Bioanal Chem 410:1991-2000.
• Pillet, F., L. Gibot, A. Catrain, J. Kolosnjaj-Tabi, K. Courtois, T. Chretiennot, E. Bellard, J. Tarayre, M. Golzio, R. Vezinet, and M. P. Rols*. (2018) High power electromagnetic pulse applicators for evaluation of biological effects induced by electromagnetic radiation waves. Rsc Adv 8:16319-16329.
• Lamarche, C., C. Da Silva, G. Demol, E. Dague, M. P. Rols*, and F. Pillet*. (2018) Electrical discharges in water induce spores' DNA damage. PLoS One 13:e0201448.
• Gui, P., M. Ben-Neji, E. Belozertseva, F. Dalenc, C. Franchet, J. Gilhodes, A. Labrousse, E. Bellard, M. Golzio, R. Poincloux, I. Maridonneau-Parini, and V. Le Cabec. (2018) The Protease-Dependent Mesenchymal Migration of Tumor-Associated Macrophages as a Target in Cancer Immunotherapy. Cancer Immunol Res 6:1337-1351.
• Figarol, A., L. Gibot*, M. Golzio, B. Lonetti, A. F. Mingotaud*, and M. P. Rols. (2018) A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers. Drug Deliv 25:1766-1778.
• Ciobanu, F., M. Golzio, E. Kovacs, and J. Teissie*. (2018) Control by Low Levels of Calcium of Mammalian Cell Membrane Electropermeabilization. J Membr Biol 251:221-228.
• Kolosnjaj-Tabi, J.*, L. Gibot, I. Fourquaux, M. Golzio, and M. P. Rols*. (2018) Electric field-responsive nanoparticles and electric fields: physical, chemical, biological mechanisms and therapeutic prospects. Adv Drug Deliv Rev DOI 10.1016/j.addr.2018.10.017

2017

• Tamra, A., D. Dubuc, M. P. Rols, and K. Grenier. (2017) Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation. IEEE T Microw Theory 65:3512-3518.
• Ric, A., V. Ong-Meang, V. Poinsot, N. Martins-Froment, F. Chauvet, A. Boutonnet, F. Ginot, V. Ecochard, L. Paquereau, and F. Couderc. (2017) ssDNA degradation along capillary electrophoresis process using a Tris buffer. Electrophoresis 38:1624-1631.
• Lechevallier, S., R. Mauricot, H. Gros-Dagnac, S. Chevreux, G. Lemercier, E. Phonesouk, M. Golzio, and M. Verelst. (2017) Silica-Based Nanoparticles as Bifunctional and Bimodal Imaging Contrast Agents. Chempluschem 82:770-777.
• Mery, E#., M. Golzio#, S. Guillermet, D. Lanore, A. Le Naour, B. Thibault, A. F. Tilkin-Mariame, E. Bellard, J. P. Delord, D. Querleu, G. Ferron, and B. Couderc. (2017) Fluorescence-guided surgery for cancer patients: a proof of concept study on human xenografts in mice and spontaneous tumors in pets. Oncotarget 8:109559-109574.
• Guillet, J. F., E. Flahaut*, and M. Golzio*. (2017) A Hydrogel/Carbon-Nanotube Needle-Free Device for Electrostimulated Skin Drug Delivery. Chemphyschem 18:2715-2723.
• Pillet, F.#, L. Gibot#, M. Madi, M. P. Rols*, and E. Dague*. (2017) Importance of endogenous extracellular matrix in biomechanical properties of human skin model. Biofabrication 9:025017
• Gibot, L., M. Golzio, and M. P. Rols*. (2017) How Imaging Membrane and Cell Processes Involved in Electropermeabilization Can Improve Its Development in Cell Biology and in Clinics. Adv Anat Embryol Cell Biol 227:107-118.

2016

• Phez, E., L. Gibot, and M. P. Rols*. 2016. How transient alterations of organelles in mammalian cells submitted to electric field may explain some aspects of gene electrotransfer process. Bioelectrochemistry 112:166-172
  Pillet, F., C. Formosa-Dague, H. Baaziz, E. Dague*, and M. P. Rols*. (2016) Cell wall as a target for bacteria inactivation by pulsed electric fields. Sci  Rep 6:19778.
• Rosazza, C., H. Deschout, A. Buntz, K. Braeckmans, M. P. Rols*, and A. Zumbusch*. (2016) Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro. Mol Ther 5:e286. 
• Josserand, V., M. Keramidas, J. Lavaud, C. Righini, J. Vollaire, E. Bellard, M. P. Rols, J. Teissie, J. L. Coll, and M. Golzio*. (2016) Electrochemotherapy guided by intraoperative fluorescence imaging for the treatment of inoperable peritoneal micro-metastases. J Control Release 233:81-87.
• Mauroy, C., I. Rico-Lattes, J. Teissie, and M. P. Rols*. (2015) Electric Destabilization of Supramolecular Lipid Vesicles Subjected to Fast Electric Pulses. Langmuir 31:12215-12222.
• Mauroy, C., P. Castagnos, J. Orio, M. C. Blache, I. Rico-Lattes, J. Teissie, M. P. Rols* and M. Blanzat*. (2015) Versatile cellular uptake mediated by catanionic vesicles: simultaneous spontaneous membrane fusion and endocytosis. Mol Pharm 12:103-110.
• Till, U., L. Gibot, C. Mingotaud, P. Vicendo, M. P. Rols, M. Gaucher, F. Violleau, and A. F. Mingotaud. (2016) Self-assembled polymeric vectors mixtures: characterization of the polymorphism and existence of synergistic effects in photodynamic therapy. Nanotechnology 27:315102.
• Till, U., L. Gibot, A. F. Mingotaud, J. Ehrhart, L. Wasungu, C. Mingotaud, J. P. Souchard, A. Poinso, M. P. Rols, F. Violleau, and P. Vicendo*. 2016. Drug Release by Direct Jump from Poly(ethylene-glycol-b-epsilon-caprolactone) Nano-Vector to Cell Membrane. Molecules 21.
• Tamra, A., M. Deburghgraeve, D. Dubuc, M. P. Rols, and K. Grenier. (2016) Microwave dielectric spectroscopy for single cell irreversible electroporation monitoring. IEEE Mtt S Int Micr
• Rosazza, C., S. H. Meglic, A. Zumbusch, M. P. Rols*, and D. Miklavcic*. (2016) Gene Electrotransfer: A Mechanistic Perspective. Curr Gene Ther 16:98-129.
• Rols, M. P., and D. Miklavcic. (2016) Editorial: Gene Transfer by Electric Fields. Curr Gene Ther. 16:73-74.
• Pillet, F., I. Marjanovic, M. Rebersek, D. Miklavcic, M. P. Rols, and T. Kotnik. (2016) Inactivation of spores by electric arcs. BMC microbiology 16:148.
• Pasquet, L., E. Bellard, M. P. Rols, M. Golzio, and J. Teissie*. (2016) Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells. Biochem Biophys Rep 7:287-294.
• Madi, M., M. P. Rols*, and L. Gibot*. (2016) Gene Electrotransfer in 3D Reconstructed Human Dermal Tissue. Curr Gene Ther 16:75-82.
• Lazar, I., E. Clement, S. Dauvillier, D. Milhas, M. Ducoux-Petit, S. LeGonidec, C. Moro, V. Soldan, S. Dalle, S. Balor, M. Golzio, O. Burlet-Schiltz, P. Valet, C. Muller, and L. Nieto. (2016) Adipocyte Exosomes Promote Melanoma Aggressiveness through Fatty Acid Oxidation: A Novel Mechanism Linking Obesity and Cancer. Cancer Res 76:4051-4057.
• Golberg A, Sack M, Teissie J, Pataro G, Pliquett U, Saulis G, Stefan T, Miklavcic D, Vorobiev E, Frey W. (2016) Energy-efficient biomass processing with pulsed electric fields for bioeconomy and sustainable development. Biotechnol Biofuels Apr 27; 9:94.
• Tozon N, Lampreht Tratar U, Znidar K, Sersa G, Teissie J, Cemazar M. (2016) Operating Procedures of the Electrochemotherapy for Treatment of Tumor in Dogs and Cats. J Vis Exp  Oct 24;(116). 
• Kamensek, U., M. P. Rols, M. Cemazar, and M. Golzio*. (2016) Visualization of Nonspecific Antitumor Effectiveness and Vascular Effects of Gene Electro-Transfer to Tumors. Curr Gene Ther 16:90-97.
• Kulbacka, J., A. Pucek, M. Kotulska, M. Dubinska-Magiera, J. Rossowska, M. P. Rols, and K. A. Wilk. (2016) Electroporation and lipid nanoparticles with cyanine IR-780 and flavonoids as efficient vectors to enhanced drug delivery in colon cancer. Bioelectrochemistry 110:19-31.
• Laurent, V., A. Guerard, C. Mazerolles, S. Le Gonidec, A. Toulet, L. Nieto, F. Zaidi, B. Majed, D. Garandeau, Y. Socrier, M. Golzio, T. Cadoudal, K. Chaoui, C. Dray, B. Monsarrat, O. Schiltz, Y. Y. Wang, B. Couderc, P. Valet, B. Malavaud, and C. Muller. (2016) Periprostatic adipocytes act as a driving force for prostate cancer progression in obesity. Nat Commun 7:10230.
• Carayon, C., A. Ghodbane, L. Gibot, R. Dumur, J. Wang, N. Saffon, M. P. Rols, K. M. Solntsev, and S. Fery-Forgues. (2016) Conjugates of Benzoxazole and GFP Chromophore with Aggregation-Induced Enhanced Emission: Influence of the Chain Length on the Formation of Particles and on the Dye Uptake by Living Cells. Small 12:6602-6612.
• Till, U., L. Gibot, P. Vicendo, M. P. Rols, M. Gaucher, F. Violleau*, and A. F. Mingotaud*. (2016) Crosslinked polymeric self-assemblies as an efficient strategy for photodynamic therapy on a 3D cell culture. Rsc Adv 6:69984-69998. 
• Rebersek, M., I. Marjanovic, S. Begus, F. Pillet, M. P. Rols, D. Miklavcic, and T. Kotnik. (2015) Generator and Setup for Emulating Exposures of Biological Samples to Lightning Strokes. IEEE Trans Biomed Eng 62:2535-2543
• Gibot, L., and M. P. Rols*. (2016) Gene transfer by pulsed electric field is highly promising in cutaneous wound healing. Expert Opin Biol Ther 16:67-77.

2015

• Madi, M., M. P. Rols*, and L. Gibot*. (2015) Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue. J Membr Biol 248:903-908.
• Luengo, E., J. M. Martinez, M. Coustets, I. Alvarez, J. Teissie, M. P. Rols*, and J. Raso*. (2015) A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris. The J Membr Biol 248:883-891.
• Frandsen, S. K., L. Gibot, M. Madi, J. Gehl*, and M. P. Rols*. (2015) Calcium Electroporation: Evidence for Differential Effects in Normal and Malignant Cell Lines, Evaluated in a 3D Spheroid Model. PLoS One 10:e0144028.
• Formosa, C., V. Lachaize, C. Gales, M. P. Rols, H. Martin-Yken, J. M. Francois, R. E. Duval, and E. Dague. (2015) Mapping HA-tagged protein at the surface of living cells by atomic force microscopy. J Mol Recogn 28:1-9.
• Coustets M, Ganeva V, Galutzov B, Teissie J*. (2015) Millisecond duration pulses for flow-through electro-induced protein extraction from E. coli and associated eradication. Bioelectrochemistry Jun; 103:82-91.
• Guionet A, David F, Zaepffel C, Coustets M, Helmi K, Cheype C, Packan D, Garnier JP, Blanckaert V, Teissié J*. (2015) E. coli     electroeradication on a closed loop circuit by using milli-, micro- and nanosecond pulsed electric fields: comparison between energy costs. Bioelectrochemistry  Jun; 103:65-73. 
• Coustets M, Joubert-Durigneux V, Hérault J, Schoefs B, Blanckaert V, Garnier JP, Teissié J*. (2015) Optimization of protein electroextraction from microalgae by a flow process. Bioelectrochemistry Jun;103:74-81. 
• Dirat, B., I. Ader, M. Golzio, F. Massa, A. Mettouchi, K. Laurent, F. Larbret, B. Malavaud, M. Cormont, E. Lemichez, O. Cuvillier, J. F. Tanti, and F. Bost. (2015) Inhibition of the GTPase Rac1 mediates the antimigratory effects of metformin in prostate cancer cells. Mol Cancer Ther 14:586-596.
• Chretiennot, T., A. Catrain, R. Vezinet, L. Gibot, F. Pillet, and M. P. Rols*. (2015) New Efficient High Power Microwave Applicators Enabling Optimal E-Field Coupling and Homogeneity into Biological Sample. IEEE Pulsed Power Conference.
• Chopinet, L., and M. P. Rols*. (2015) Nanosecond electric pulses: a mini-review of the present state of the art. Bioelectrochemistry 103:2-6.
• Ceolato, R., M. Golzio, C. Riou, X. Orlik, and N. Riviere. (2015) Spectral degree of linear polarization of light from healthy skin and melanoma. Opt Express 23:13605-13612.
• Chabot, S., J. Teissie, and M. Golzio*. (2015) Targeted electro-delivery of oligonucleotides for RNA interference: siRNA and antimiR. Adv Drug Deliv Rev 81:161-168.

2014

• Guionet A, Joubert-Durigneux V, Packan D, Cheype C, Garnier JP, David F, Zaepffel C, Leroux RM, Teissié J, Blanckaert V. (2014) Effect of nanosecond pulsed electric field on Escherichia coli in water: inactivation and impact on protein changes. J Appl Microbiol 117:721-8.
• Ader, I., C. Gstalder, P. Bouquerel, M. Golzio, G. Andrieu, S. Zalvidea, S. Richard, R. A. Sabbadini, B. Malavaud, and O. Cuvillier. (2015) Neutralizing S1P inhibits intratumoral hypoxia, induces vascular remodelling and sensitizes to chemotherapy in prostate cancer. Oncotarget 6:13803-13821.
• Ganeva V, Galutzov B, Teissie J*. (2014) Evidence that pulsed electric field treatment enhances the cell wall porosity of yeast cells. Appl. Biochem Biotechnol Feb;172(3):1540-52.
• Wasungu, L., F. Pillet, E. Bellard, M. P. Rols, and J. Teissie*. (2014) Shock waves associated with electric pulses affect cell electro-permeabilization. Bioelectrochemistry 100:36-43.
• Vezinet, R., A. Catrain, T. Chretiennot, J. Tarayre, L. Gibot, F. Pillet, and M. P. Rols. (2014) Plane Wave in Vitro Exposure of Biological Samples, Geometries Considerations. IEEE Int Power Modul:41-44
• Castagnos, P., I. Rico-Lattes, M. Blanzat, C. Mauroy, J. Orio, M. C. Blache, J. Teissie, and M. P. Rols. (2014) Versatile cellular uptake mediated by catanionic vesicles: simultaneous spontaneous membrane fusion and endocytosis. Molecular pharmaceutics
• Escoffre, J. M., M. Hubert, J. Teissie, M. P. Rols, and C. Favard. (2014) Evidence for electro-induced membrane defects assessed by lateral mobility measurement of a GPi anchored protein. Eur Biophys J 43:277-286 43:277-286
• Pasquet, L., E. Bellard, M. Golzio, M. P. Rols, and J. Teissie. (2014) A Double-Pulse Approach For Electrotransfection. J Membr Biol
• Escoffre JM, Bellard E, Faurie C, Sébaï SC, Golzio M, Teissié J, Rols MP. (2014)Membrane disorder and phospholipid scrambling in electropermeabilized and viable cells. Biochim Biophys Acta1838:1701-1709
• Gibot L, Lemelle A, Till U, Moukarzel B, Mingotaud AF, Pimienta V, Saint-Aguet P, Rols MP, Gaucher M, Violleau F, Chassenieux C, Vicendo P. (2014) Polymeric Micelles Encapsulating Photosensitizer: Structure/Photodynamic Therapy Efficiency Relation. Biomacromolecules 15:1443-1455
• Martínez O, Bellard E, Golzio M, Mechiche-Alami S, Rols MP, Teissié J, Ecochard V, Paquereau L. (2014) Direct Validation of Aptamers as Powerful Tools to Image Solid Tumor. Nucleic Acid Ther24:217-225
• Golzio, M. and Teissie J. (2014). siRNA delivery via electropulsation: a review of the basic processes. Methods Mol Biol 1121: 81-98
• Teissie, J. and Golzio M. (2014). Direct imaging of siRNA electrotransfer at the single-cell level.Methods Mol Biol 1121: 121-130

2013

• Rosazza C, Buntz A, Rieß T, Wöll D, Zumbusch A, Rols MP. (2013) Intracellular tracking of single-plasmid DNA particles after delivery by electroporation. Mol Ther. 21(12):2217-26
• Orio J, Bellard E, Baaziz H, Pichon C, Mouritzen P, Rols MP, Teissié J, Golzio M, Chabot S. (2013) Sub-cellular temporal and spatial distribution of electrotransferred LNA/DNA oligomer. J RNAi Gene Silencing 9:479-85
• Chabot S, Pelofy S, Teissié J, Golzio M. (2013) Delivery of RNAi-Based Oligonucleotides by Electropermeabilization. Pharmaceuticals (Basel) 10;6(4):510-21
• Chopinet L, Batista-Napotnik T, Montigny A, Rebersek M, Teissié J, Rols M-P, Miklavčič D. (2013) Nanosecond electric pulses effects on gene expression. Journal of Membrane Biology 246(11):851-9
• Gibot L, Auger FA, Lacroix D. (2013) The Pivotal Role of Vascularization in Tissue Engineering.Annu Rev Biomed Eng In press
• Gibot L, Rols MP. (2013) 3D Spheroids’ Sensitivity to Electric Field Pulses Depends on Their Size. J Membr Biol 246(10):745-50
• Chopinet L, Roduit C, Rols MP, Dague E. (2013) Destabilization induced by electropermeabilization analyzed by atomic force microscopy. Biochim Biophys Acta 1828(9):2223-9
• Gibot L, Rols MP. (2013) Progress and Prospects: The Use of 3D Spheroid Model as a Relevant Way to Study and Optimize DNA Electrotransfer. Curr Gene Ther 13(3):175-81
• Chabot S, Rosazza C, Golzio M, Zumbusch A, Teissié J, Rols MP. (2013) Nucleic acids electro-transfer: from bench to bedside. Curr Drug Metab 14(3):300-8
• Chopinet L, Formosa C, Rols MP, Duval RE, Dague E. (2013) Imaging living cells surface and quantifying its properties at high resolution using AFM in QI™ mode. Micron 48:26-33
• Gibot L, Wasungu L, Teissié J, Rols MP. (2013) Antitumor drug delivery in multicellular spheroids by electropermeabilization. J Control Release 167(2):138-147
• Chabot S, Orio J, Schmeer M, Schleef M, Golzio M, Teissié J.(2013) Minicircle DNA electrotransfer for efficient tissue-targeted gene delivery. Gene Ther 20(1):62-8

2012

• Bellard E, Markelc B, Pelofy S, Le Guerroué F, Sersa G, Teissié J, Cemazar M, Golzio M. (2012) Intravital microscopy at the single vessel level brings new insights of vascular modification mechanisms induced by electropermeabilization. J Control Release 163(3):396-403
• Chabot S, Rosazza C, Golzio M, Zumbush A, Teissié J, Rols MP. (2012) Nucleic Acids Electro-transfer: From Bench to Bedside. Curr Drug Metab.
• Portet T, Mauroy C, Démery V, Houles T, Escoffre JM, Dean DS, Rols MP. (2012) Destabilizing giant vesicles with electric fields: an overview of current applications. J Membr Biol. 245(9):555-64.
• Escoffre JM, Nikolova B, Mallet L, Henri J, Favard C, Golzio M, Teissie J, Tsoneva I, Rols MP. (2012) New insights in the gene electrotransfer process: evidence for the involvement of the plasmid DNA topology. Curr Gene Ther. 12(5):417-22
• Mauroy C, Portet T, Winterhalder M, Bellard E, Blache MC, Teissié J, Zumbusch A, Rols MP. (2012) Giant lipid vesicles under electric field pulses assessed by non invasive imaging.Bioelectrochemistry. 87:253-9
• Pelofy S, Teissié J, Golzio M, Chabot S. (2012) Chemically modified oligonucleotide-increased stability negatively correlates with its efficacy despite efficient electrotransfer. J Membr Biol.245(9):565-71
• Chabot S, Bellard E, Nielsen SJ, Orio J, Castanier R, Golzio M, Teissié J. (2012) LNA-based oligonucleotide electrotransfer for miRNA inhibition. Mol Ther. 20(8):1590-8
• Escoffre JM, Rols MP. (2012) Electrochemotherapy: Progress and Prospects. Curr Pharm Des18(23):3406-15
• Mauroy C, Castagnos P, Blache MC, Teissié J, Rico-Lattes I, Rols MP, Blanzat M. (2012) Interaction between GUVs and catanionic nanocontainers: new insight into spontaneous membrane fusion.Chem Commun 48(53):6648-50
• Golzio M, Escoffre JM, Teissié J. (2012) shRNA-mediated gene knockdown in skeletal muscle.Methods Mol Biol 798:491-501
• Teissié J, Escoffre JM, Paganin A, Chabot S, Bellard E, Wasungu L, Rols MP, Golzio M. (2012) Drug delivery by electropulsation: Recent developments in oncology. Int J Pharm 423(1):3-6
• Rosazza C, Phez E, Escoffre JM, Cézanne L, Zumbusch A, Rols MP. (2012). Cholesterol implications in plasmid DNA electrotransfer: Evidence for the involvement of endocytotic pathways.Int J Pharm 423(1):134-43
• Tamzali Y, Borde L, Rols MP, Golzio M, Lyazrhi F, Teissie J. (2012). Successful treatment of equine sarcoids with cisplatin electrochemotherapy: A retrospective study of 48 cases. Equine Vet J44(2):214-20

Download the complete list and the collaborative publications from 2016 to 2005

Valorisation

3rd Price of the 25th "concours innovation Midi-Pyrénées" , Section « laboratory and research teams » :

• J.TEISSIE, MP. ROLS, M. GOLZIO, Y. TAMZALI, B.COUDERC. Device for eradication of horses cutaneous tumors by electrochemo- and electrogenotherapy.

Application for Technology Transfer : « Fluorescence imaging device for small animals (whole body)» Date of the comity : 20 Avril 2006. N° AV ou N° St DI 00745-01

• The invention is a device which allows the study, by fluorescence imaging, of few centimeters objects. The inventive activity does not allow to apply for a patent : this is a combination of well-known elements that could be evident for an expert. However, this could be a skill, specially in micro biology.

• The "Comité des Engagements" gave a favorable verdict to Technology transfer via "Licence de Savoir-Faire".

Patents

FR 1257125 (2012-07-23) SYSTEME DE DELIVRANCE A BASE DE LECTINE

Inventors :

• LADURANTIE, Caroline; (FR).
• GROSS, Grégory; (FR).
• PAQUEREAU, Laurent; (FR).

(WO/2004/033491) PROTEINES INSECTICIDES ET/OU NEMATICIDES

Inventors :

• CAYLEY, Patricia, Jane; (GB).
• FOURNIER, Didier; (FR).
• MACKAY, Elaine, Anne; (GB).
• MACKAY, Katrina; (GB).
• ONISKO, Bruce, Charles; (US).
• PAQUEREAU, Laurent; (FR).

(WO/2001/000840) PROTEINES INSECTICIDES ET NEMATICIDES

Inventors:

• FOURNIER, Didier; (FR).
• PAQUEREAU, Laurent; (FR).
• KLAEBE, Alain; (FR).
• CHAVANT, Louis; (FR).

Collaborations

International

• University of Konstanz, Germany (Pr A. Zumbush)
• Herlev Hospital, Denmark (Pr J. Gehl and Dr S. Krog-Fransden)
• Medical University of Worklaw, Poland (Dr J. Kulbata)
• University Campus Bio-Medico of Rome, Italy (Pr E Signori)
• University of Ljubljana, Slovenia (Pr D. Miklavcic, Pr M. Cemazar)
• Ruđer Bošković Institute Zagreb, Croatia (Pr N. Nadica Ivosevic DeNardis)

National

• CEA Gramat (Dr R. Vézinet)
• ITHPP Thegra (G. Demol, C. Lamarche)
• XLIM Limoges (Dr P. Levêque)
• Institut Curie, Paris (Dr C. Lamaze)
• Inria, Bordeaux (Dr C. Poignard)
• CHU Bondy, Paris (Pr O. Seror)
• IAB Grenoble (Dr JL Coll)

Toulouse

• GTP Technology (Dr E. Devic)
• Urosphere (Dr S. Chabot)
• IMRCP (Pr F. Couderc)
• IUCT (Dr G. Ferron)
• LMGM (Dr P. Rousseau)
• LSPCMIB (Dr JM Escudier)
• LAAS (Drs Dague, Dubuc, Grenier)
• ITAV (Prs B. Ducommun)
• LCC (Pr I. Malfant, Dr L. Valade)
• IMRCP (Dr P Vicendo)
• Laplace (Pr N Merbahi)
• CPTP (Dr S. Guerder)
• CIRIMAT (Dr E. Flahaut)
• ENVT (Pr Y. Tamzali)

IPBS

• A Milon, O Cuvillier, C Muller, I Maridonneau-Parini

Financial supports

Industry

• Urosphere company 
• GTP Technology company 
• ITHPP company

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Public grants

• Institut National du Cancer (INCA)
• Agence Nationale de la Recherche (ANR)
• Centre National de la Recherche Scientifique (CNRS)
• Fondation ARC
• Direction Générale des Armées (DGA)
• Direction Générale des Entreprise (DGE)
• Institut national de la santé et de la recherche médicale (INSERM)
• Région Occitanie (COMUE)
• SATT-ToulouseTechTransfert 
• Canceropole GSO
• Région Midi-Pyrénées
• Fondation J. Paufigue (SILAB)
• Cifre fellowships