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Julien Marcoux

Julien Marcoux

 

Personal Statement

My research interests are focused on using mass spectrometry as a tool for studying soluble and membrane associated protein assemblies.

During my time as a Ph.D. student I applied mass spectrometry to unravel the structural mechanism of activation of the NADPH oxidase from neutrophils. The project also gave me the opportunity to gain experimental experience in various biophysical techniques as well as in molecular biology, protein expression and purification. I expanded and developed these skills during my two postdoctoral positions in Oxford and Strasbourg, by applying them to significantly larger soluble complexes and membrane proteins. I obtained, in 2015, a CNRS Research Associate position at the Institute of Pharmacology and Structural Biology in Toulouse, where I am currently developing structural MS-based methods for the analysis of supramolecular complexes, including the 20S human proteasome and its regulators.

 

Visit our website to learn more about our team, our projects and developed structural MS methods.

 

 

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Current Position

Researcher at CNRS (French National Center for Scientific Research) at the Institute of Pharmacology and Structural Biology (IPBS-UMR5089), 205 route de Narbonne, 31077 Toulouse cedex 04, FRANCE

Education - Training

  • 2003-2006 : Biochemistry Engineer of the National Institute of Applied Sciences in Lyon
  • 2005-2006 : Master in Structural and Functional Biochemistry of the University Claude Bernard Lyon 1 / 6-month placement in a Mexican Oceanographic Centrer (Ensenada, Baja California, Mexico) to study the production and analysis of superoxide dismutase extracted from microalgae cultivated in a photo-bioreactor
  • 2006-2010 : PhD in Structural Biology of the University Joseph Fourier of Grenoble (France), Laboratory : Institute of Stuctural Biology (Dr Eric Forest and Prof. Franck Fieschi) Subject: Structural and functional analysis of neutrophil NADPH oxidase: use of mass spectrometry to characterize p47phox conformational changes upon activation
  • 2011-2014 : Postdoctoral fellow at the University of Oxford (Chemistry Departement) (UK) – Laboratory : Physical and Theoretical Chemistry Laboratory (Prof. Dame Carol Robinson) - Subject : Use of native MS and Ion-Mobility to characterize soluble and membrane protein complexes.
  • 2014-2015 : Postdoctoral fellow at University of Strasbourg (France) - Laboratory : BioOrganic Mass Spectrometry Laboratory (Dr Sarah Sanglier-Cianférani) - Subject : Use of structural MS to investigate monoclonal antibodies and other protein complexes.
  • 2015 : Permanent researcher position at CNRS

Scientific achievements

My scientific achievements consist of 46 research articles, accumulating a citation index of 1776 with an h-index of 22, according to Google Scholar

Main selected publications (Top 10 since 2015):

  • Lesne J*, Locard-Paulet M*, Parra J, Zivković D, Menneteau T, Bousquet MP, Burlet-Schiltz O, Marcoux J (2020) “Conformational maps of human 20S proteasomes reveal PA28- and immuno-dependant inter-ring crosstalks” Nature Communications 11(1):6140
  • Shayan R, Rinaldi D, Larburu N, Plassart L, Balor S, Bouyssié D, Lebaron S, Marcoux J*, Gleizes PE*, Plisson-Chastang C* (2020)  “Good vibrations : structural remodeling of maturing yeast pre-40S ribosomal particles followed by cryo-electron microscopy” Molecules 25(5), 1125.
  • Bouyssié D*, Lesne J*, Locard-Paulet M, Albigot R, Schiltz O, Marcoux J (2019) “HDX-Viewer: interactive 3D visualization of Hydrogen-Deuterium eXchange data” BioInformatics 35(24):5331-5333.
  • Guillet V, Bordes P, Bon C, Marcoux J, Gervais V, Julie Sala A, Dos Reis S, Slama N, Mares-Mejía I, Cirinesi AM, Genevaux P, Mourey L (2019)Structural insights into chaperone addiction of toxin-antitoxin systems” Nature Communications 10(1):782.
  • Locard-Paulet M, Parra J, Albigot R, Mouton-Barbosa E, Bardi L, Burlet-Schiltz O, Marcoux J (2018) “VisioProt-MS: interactive 2D maps from intact protein mass spectrometry” BioInformatics In Press 35(4):679-681
  • Carel C*, Marcoux J*, Parra J, Réat V, Latgé G, Laval F, Burlet-Schiltz O, Demange P, Milon A, Daffé M, Tropis M, Renault M (2017) “Post-translational O-mycoloylation mediates protein targeting to the mycomembrane in C. glutamicumProceedings of the National Academy of Sciences 114(16) : 4231-4236
  • Parra J*, Marcoux J*, Poncin I, Canaan S, Herrmann JL, Nigou J, Burlet-Schiltz O, Rivière M (2017) “Scrutiny of Mycobacterium tuberculosis 19kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm” Scientific Reports 7:43682
  • Mehmood S, Marcoux J, Gault J, Quigley A, Michaelis S, Young SG, Carpenter EP, Robinson CV (2016) “Mass spectrometry captures off-target drug binding and provides mechanistic insights into the human metalloprotease ZMPSTE24” Nature Chemistry 8(12) : 1152-1158
  • Marcoux J, Mangione PP, Porcari R, Degiacomi M, Verona G, Taylor GW, Giorgetti S, Raimondi S, Sanglier-Cianférani S, Benesch JL, Cecconi C, Naqvi MM, Gillmore JD, Hawkins PN, Stoppini M, Robinson CV, Pepys MB, Bellotti V (2015) “A novel mechano-enzymatic cleavage mechanism underlies transthyretin amyloidogenesis” EMBO Molecular Medicine 7(10) : 1337-1349
  • Marcoux J, Cianférani S (2015) “Towards integrative structural mass spectrometry: benefits from hybrid approaches” Methods 89:4-12.

Fundings

  • Excellence Initiative Grant (IdEx) (2014)
  • ANR Young Researchers (2019-2022). ProteasoRegMS: Structural Mass Spectrometry to study the interplay between proteasome complexes regulation mechanisms
  • INCA (2020-2024). ONCORIB: A structural, compositional and functional characterization of mutant ribosomes (collaboration Celia Plisson-Chastang, CBI Toulouse)

Prize

CNRS Bronze Medal (2020)

Media

Google Scholar, ResearchGateLinkedInORCIDPublons

Alumni

  • Manoel André / Master 2 Student (2021)
  • Angelique Dafun Sanchez / PhD Student (2020-2023)
  • Titaua Chabanne / Master 2 Student (2020)
  • Sandrine Lise / Master 2 Student (2018/2019)
  • Dusan Zivkovic / PhD Student (2017-2021)
  • Jean Lesne / Master 2 Student (2017-2018)
  • Dusan Zivkovic / Master 2 Student (2016-2017)
  • Efthymia Ioanna Koufogeorgou / Master 2 Student (2015-2016)
  • Julien Parra / Post-Doctoral Fellow (2015-2018)
  • Frédéric Rivière / Master 2 Student (2015)