Most mycobacteria are environmental species, causing disease only occasionally when they encounter a susceptible human or animal host. A few species, such as Mycobacterium tuberculosis, have become major pathogens during the course of evolution, and have acquired the ability to persist in their host despite natural or vaccine-induced immunity. Recent genomic studies by our lab and others provided evidence that early episodes of horizontal transfer of genomic islands from surrounding environmental species likely contributed to the evolution of M. tuberculosis towards a sophisticated human-adapted pathogen. We are currently deciphering the function of several of these genomic islands, including toxin-antitoxin systems, in mycobacterial metabolism and pathogenicity.
In addition to comparative genomics, we exploit global gene expression profiling of host cell and mycobacterial responses to infection. Dual host and pathogen DNA microarray analyses allowed to identify several families of eukaryotic and microbial genes whose expression is modulated upon infection. In particular, we recently identify a novel mechanism of innate immune control of pathogens through zinc intoxication, and resistance strategies in pathogenic mycobacteria involving P-ATPases were discovered. We are currently deciphering the molecular and cellular mechanisms involved in microbial poisoning by zinc in infected macrophages, and the function of several P-ATPases in M. tuberculosis physiology and virulence. We also use dual RNA-seq analysis and Tn-seq analysis to understand M. tuberculosis adaptation to various host-imposed stresses, including hypoxia, and the role of various metabolic circuits in mycobacterial response to stress.
Matthias Wilmanns EMBL, Hamburg, Germany Visit the Wilmanns lab webpage
Luiz Pedro de Carvalho The Francis Crick Institute, London, UK Visit the Wilmanns lab webpage
Patrice Catty Laboratoire de Chimie et Biologie des Métaux, Grenoble Visit the Michaud-Soret lab webpage
- Freire*, Gutierrez* et al. 2019 An NAD+ phosphorylase toxin triggers Mycobacterium tuberculosis cell death Mol Cell
- Levillain*, Poquet* et al. 2017 Horizontal acquisition of a hypoxia-responsive molybdenum cofactor biosynthesis pathway contributed to Mycobacterium tuberculosis pathoadaptation PLOS Pathog
- Gouzy et al. 2014 Nitrogen metabolism in Mycobacterium tuberculosis physiology and virulence Nat Rev Microbiol
- Gouzy et al. 2014 Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection PLOS Pathog
- Gouzy et al. 2013 Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate Nat Chem Biol
- Botella et al. 2011 Mycobacterial P1-type ATPases mediate resistance to zinc poisoning in human macrophages Cell Host Microbe
- Jang et al. 2008 Horizontally acquired genomic islands in the tubercle bacilli Trends Microbiol
- Becq et al. 2008 Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli Mol Biol Evol