Cellular imaging

FV1000 confocal

Tumor cells cluster actine cytosqueleton in displayed magenta, dapi stained nucleus @Stephanie Dauvillier (CNRS), MULLER team

Spining Disk

Extract of time lapse of Human airway organoids (AOs) control (up) & infected with dTomato-expressing Mycobacterium abscessus (bottom). @MEUNIER team

LSM710 confocal

Lipid droplets in breast tumor cells. (lipids in green, Actine in red & nucleus in blue) ©Marie Rebeaud (CNRS), MULLER team

LSM710 confocal

Protein relocalisation after DNA double strand damage induced with biphoton laser. ©Phillipe Frit (CNRS), BRITTON team

Axio Imager M2 Zeiss

Grass blade autofluorescence Widefield and with Apotome module (7µm section) ©TRI: Eve Pitot (CNRS)

FV1000 Widefield

Grass blade autofluorescence Widefield & after deconvolution ©TRI: Eve Pitot (CNRS)

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Cellular imaging allows high-resolution visualization of cellular and subcellular structures either in fixed or live specimens. For this purpose, our core facility offers wide field and confocal microscopy equipment to perform such type of analysis in a standard or BSL3 environment for the use of human pathogens.

In particular, we offer the possibility to perform FLIM and FRAP techniques in live specimens using a confocal laser scanning microscope equipped with femtosecond pulsed laser.

Confocal & Multiphoton Zeiss 710 NLO « Spectral »

Confocal & Widefield Olympus FV1000

Widefield & Apotome Axio Imager M2 Zeiss

Spinning disk Andor/Olympus