IPBS-Toulouse

Creation of a CNRS Joint international laboratory headed by C. Vérollet and G. Lugo-Villarino

The International laboratory MAC-TB/HIV has just been recognized as a CNRS International Research Partnership (IRP) for a 5-year period (2021-2025).

MAC-TB/HIV – Understanding how tuberculosis-associated microenvironment increases susceptibility to HIV-1 infection: a perspective on metabolism and activated macrophages.

This IRP takes place in the context of co-infection, where tuberculosis (TB) and acquired immune deficiency syndrome (AIDS) are among the deadliest diseases due to a single infectious agent. The well-known synergy between the two pathogens responsible for these diseases, Mycobacterium tuberculosis (Mtb) and HIV-1, respectively, places an immense burden on the health care systems in resource-limited countries where co-infection with these pathogens is prevalent, but also a serious public health issue in South American and European countries. The central theme of MAC-TB/HIV is that TB-associated microenvironments modulate the metabolic and activated state of human macrophages through dysregulation of lipid networks, transforming these cells into a Trojan horse for HIV-1 dissemination. This IRP consortium is already experiencing success in terms of: i) increasing the synergy between all teams and personnel involved, ii) creating a dynamic pipeline between the scientific aims shared by French and Argentinian teams, iii) producing quality scientific output in terms of publications, and iv) gaining visibility with the international, national and local news media.

 

The IRP of the CNRS aim to consolidate international research partnerships. The MAC-TB/HIV laboratory has been created for a 5-year period (2021-2025) and involves 3 groups within IPBS headed by Drs. O. Neyrolles, C. Vérollet and R. Poincloux, and the Laboratorio de Inmunología de Enfermedades Respiratorias led by Drs. MDC. Sasiain and L. Balboa at the IMEX CONICET- Academia Nacional de Medicina of Buenos Aires (Argentina).

Publications

  • Vahlas Z, Faivre Nψ, Monard SC, Hertel Q, Maio M, Barros J, Ben Neji M, Lucas A, Manh TV, Argüello RJ, Corti M, Poincloux R, Blanchet F, Raynaud-Messina B, Lugo Villarino G, Neyrolles O, Balboa L*, Verollet C*. Tuberculosis-induced glycolysis favors tunneling nanotube formation, promoting HIV-1 dissemination in macrophages. In preparation.
  • Maio M, Joly M, Vahlas Z, Barros J, Marín Franco J, Genoula M, Monard SC, Vecchione M, Fuentes F, Polo Virginia G, Quiroga M, Vermeulen M, Argüello RJ, Inwentarz S, Musella R, Ciallella L, Montaner P González, Palmero D, Lugo Villarino G, del Carmen Sasiain M, Neyrolles O, Verollet Cψ*, Balboa L*. Elevated glycolytic metabolism of monocytes limits the generation of HIF-1α-driven migratory dendritic cells in tuberculosis. Elife. 2023 12:RP89319. In revision process. https://doi.org/10.7554/eLife.89319.1
  • Mascarau R, Woottum M, Fromont L, Gence R, Lévêque K, Bertrand F, Beunon T, Métais A, Cantaloube-Ferrieu V, El Costa H, Jabrane-Ferrat N, Gallois Y, Guibert N, Davignon JL, Favre G, Lagane B, Maridonneau-Parini I, Poincloux R, Bénichou S, Raynaud-Messina B*, Vérollet C*. Productive HIV-1 infection of tissue macrophages by fusion with infected CD4+ T cells. J Cell Biol. 2023
  • Dupont M, Rousset S, Manh TV, Monard SC, Pingris K, Souriant S, Vahlas Z, Velez T, Poincloux R, Maridonneau-Parini I, Neyrolles O, Lugo-Villarino G*, Vérollet C*. Dysregulation of the IFN-I signaling pathway by Mycobacterium tuberculosis leads to exacerbation of HIV-1 infection of macrophages. J Leukoc Biol. 2022 May 19.
  • Bernard-Raichon L, Colom A, Monard SCψ, Namouchi A, Cescato M, Garnier H, Leon-Icaza SA, Métais A, Dumas A, Corral D, Ghebrendrias N, Guilloton P, Vérollet C, Hudrisier D, Remot A, Langella P, Thomas M, Cougoule C, Neyrolles O*, Lugo-Villarino G*. A Pulmonary Lactobacillus murinus Strain Induces Th17 and RORγt+ Regulatory T Cells and Reduces Lung Inflammation in Tuberculosis. J Immunol. 2021 Oct ;207(7):1857-1870.
  • Genoula M, Marín Franco JL, Maio M, Dolotowicz B, Ferreyra M, Milillo A, Mascarau R, Moraña EJ, Palmero D, Fuentes F, López B, Barrionuevo P, Neyrolles O, Cougoule C, Lugo-Villarino G, Vérollet C, Sasiain MdC and Balboa L. Mycobacterium tuberculosis Modulates the Metabolism of Alternatively Activated Macrophages to Promote Foam Cell Formation and Intracellular Survival. PLOS Pathog, 2020 Oct 1;16(10):e1008929.
  • Marín Franco JL, Genoula M, Corral C, Duette G, Ferreyra M, Maio M, Dolotowicz MB, Omar Aparicio-Trejo E, Patiño-Martínez E, Fuentes F, Soldan V, José Moraña E, Palmero D, Ostrowski M, Schierloh P, Sánchez-Torres C, Hernández-Pando R, Pedraza-Chaverri J, Rombouts Y, Layre E, Hudrisier D, Vérollet C, Neyrolles O, Sasiain MdC, Lugo-Villarino G*, and Balboa L*. Host-derived lipids from pleural effusions of TB patients impair anti-mycobacterial functions in human macrophages though HIF-1α-mediated metabolic reprogramming. Cell Rep. 2020 Dec 29;33(13):108547.
  • Dupont M, Souriant S, Balboa L, Vu Manh TP, Pingris P, Rousset S, Cougoule C, Rombouts Y, Poincloux Y, Ben Neji M, Palmero D, Corti M, Benet Garrabé S, Allers C, Kaushal D, Kuroda MJ, Martinez-Picado J, Izquierdo-Useros N, Sasiain MdC, Maridonneau-Parini I*, Neyrolles O*, Vérollet C* and Lugo-Villarino G*. Tuberculosis-associated IFN-I induces Siglec-1 on microtubule-containing tunneling nanotubes and favors HIV-1 spread in macrophages. Elife. 2020, 9:e52535. DOI: 10.7554/eLife.52535
  • Souriant S, Balboa L, Pingris K, Kviatcovsky D, Cougoule C, Lastrucci C, Bah A, Gasser R, Poincloux R, Raynaud-Messina B, Al Saati T, Inwentarz S, Poggi S, Moraña EJ, Gonzalez-Montaner P, Corti M, Lagane B, Vergne I, Allers C, Kaushal D, Kuroda MJ, Sasiain MDC, Neyrolles O*, Maridonneau-Parini I*, Lugo-Villarino G*, Vérollet C*. Tuberculosis exacerbates HIV-1 infection through IL-10/STAT3-dependent tunneling nanotube formation in macrophages. Cell Rep. 2019, 26(13):3586-3599.e7. DOI: 10.1016/j.celrep.2019.02.091
  • Bénard A, Sakwa I, Schierloh P, Colom A, Mercier I, Tailleux L, Jouneau L, Boudinot P, Al-Saati T, Lang R, Rehwinkel J, Loxton AG, Kaufmann SHE, Anton-Leberre V, O’Garra A, Sasiain MDC, Gicquel B, Fillatreau S, Neyrolles O, Hudrisier D. B Cells Producing Type I IFN Modulate Macrophage Polarization in Tuberculosis. Am J Respir Crit Care Med. 2018 Mar 15;197(6):801-813. DOI: 10.1164/rccm.201707-1475OC
  • Colado A, Genoula M, Cougoule Cψ, Marín Franco JL, Almejún MB, Risnik D, Kviatcovsky D, Podaza E, Elías EE, Fuentes F, Maridonneau-Parini I, Bezares FR, Fernandez Grecco H, Cabrejo M, Jancic C, Sasiain MDC, Giordano M, Gamberale R, Balboa L, Borge M. Effect of the BTK inhibitor ibrutinib on macrophage- and γδ T cell-mediated response against Mycobacterium tuberculosis. Blood Cancer J. 2018 Nov 5;8(11):100. DOI: 10.1038/s41408-018-0136-x
  • Lugo-Villarino G*, Troegeler A*, Balboa L, Lastrucci C, Duval C, Mercier I, Bernard A, Capilla F, Al Saati T, Poincloux R, Kondova I, Verreck F, Cougoule C, Maridonneau-Parini I, Sasian MDC, Olivier Neyrolles. The C-type lectin receptor DC-SIGN has an anti-inflammatory role in human M(IL-4) macrophages in response to Mycobacterium tuberculosis. Front Immunol. 2018, 9: 1123. DOI: 10.3389/fimmu.2018.01123 
  • Genoula M, Marín Franco JL, Dupont M, Kviatcovsky D, Milillo A, Schierloh P, Moraña EJ, Poggi S, Palmero D, Mata-Espinosa D, González-Domínguez E, León Contreras JC, Barrionuevo P, Rearte B, Córdoba Moreno MO, Fontanals A, Crotta Asis A, Gago G, Cougoule C, Neyrolles O, Maridonneau-Parini I, Sánchez-Torres C, Hernández-Pando R, Vérollet C*, Lugo-Villarino G*, Sasiain MDC*, Balboa L. Formation of Foamy Macrophages by Tuberculous Pleural Effusions Is Triggered by the Interleukin-10/Signal Transducer and Activator of Transcription 3 Axis through ACAT Upregulation. Front Immunol. 2018, 9: 459. DOI: 10.3389/fimmu.2018.00459
  • Lastrucci C, Bénard A, Balboa L, Pingris K, Lendoiro N, Al Saati T, Poincloux R, Marion E, Kondova I, Verreck FA, Sasiain MC, Neyrolles O, Maridonneau-Parini, Lugo-Villarino G*, Cougoule C*. Tuberculosis is associated with expansion of a motile, permissive and immunomodulatory CD16(+) monocyte population via the IL-10/STAT3 axis. Cell Res. 2015, 25(12): 1333-51. DOI: 10.1038/cr.2015.123
  • Balboa L, Barrios-Payan J, González-Domínguez E, Lastrucci C, Lugo-Villarino G , Mata-Espinoza D, Schierloh P, Kviatcovsky D, Neyrolles O, Maridonneau-Parini I, Sánchez-Torres C, Sasiain Mdel C, Hernández-Pando R. Diverging biological roles among human monocyte subsets in the context of tuberculosis infection. Clin Sci. 2015, 129(4): 319-30. DOI: 10.1042/CS20150021
  • Lugo-Villarino G, Cougoule C, Meunier E, Rombouts Y, Vérollet C, Balboa L. The Mononuclear Phagocyte System in Infectious Disease. Front Immunol. 2019, 10:1443. DOI: 10.3389/fimmu.2019.01443 (Editorial)

 

Creation of a CNRS Joint international laboratory headed by C. Vérollet and G. Lugo-Villarino