Integrated Screening Platform of Toulouse (PICT)

Virginie Nahoum
Virginie Nahoum

Head of facility

PICT is a multidisciplinary platform which aims to provide the scientific and medical community with a complete package of technologies and expertise allowing the random or structure-based identification and the rational design of inhibitors of therapeutic targets, or effectors of any other target, the discovery of new enzymes and the characterization of their interactions.

PICT provides a complete package of technologies and expertise for the screening and rational design of ligands of any target and for the enzyme engineering.

PICT combines the know-how and the expertise of three facilities located in Toulouse: The IPBS (Institute of Pharmacology and Structural Biology: biophysics, structural biology and bioinformatics), which is the coordinator site, the SPCMIB (laboratory of Synthesis and Physico-Chemistry of Molecules of Biological Interest: medicinal chemistry) and the TBI (Toulouse Biotechnology Institute: enzyme discovery and optimization). These facilities conduct cutting-edge research in their own fields and dedicate technological know-how, equipment and staff to the platform.

Scientific offer

PICT provides its users with access to the tools and scientific expertise necessary for the determination of macromolecular structures, the screening and the measurement of interactions by biophysical methods, the chemical synthesis for the development of libraries of focused compounds and the optimization of hit-to-lead ligands. PICT also provides analysis, purification and identification of various families of molecules and proteins of low molecular weight, protein engineering, directed evolution and high-throughput screening of optimized enzymes, and finally, functional metagenomics for the discovery of new enzymes.

Equipment

The IPBS site focuses on peptide synthesis, on the identification of ligands using biophysical screening technologies and on the biophysical characterization of proteins and protein-ligands complexes (Dynamic Light Scattering, Size Exclusion Chromatography coupled with Multi-Angle Light Scattering, Isothermal Titration Calorimetry, Differential Scanninf Fluorimetry (DSF) and Nano-DSF, Microscale Thermophoresis, Spectral Shift). It also provides structural biology using NMR (500 MHz, 600 MHz cryoprobe and 700 MHz) and/or X-ray crystallography (crystallization and visualization robots) and bioinformatics (224-core and 40-core clusters). An in-house fragment library (about 1000 compounds) has been developed for Fragment-Based Ligand Discovery projects, and the platform also offers access to libraries of “drug-like” compounds (>11000) and peptides (>20000).

Activity

PICT is part of the regional network GenoToul and has received the national IBiSA label. The platform has been certified ISO-9001 since 2012 and NFX50-900 since 2015. This certifies the quality of services offered, with an emphasis on process control, accountability, traceability, and customer satisfaction. PICT is open to academic and government agencies as well as to companies at both the local and national levels. Various kinds of services are offered ranging from access to specialized equipment to a complete service delivery, under collaborative contracts and industrial partnerships.

Team

Scientists

Andrew Atkinson (CNRS)
Laurent Maveyraud (University)
Lionel Mourey (CNRS)
Olivier Saurel (CNRS)

Research Assistants

Nathalie Doncescu (CNRS)
Valérie Guillet (CNRS)
Virginie Nahoum (CNRS)
Pascal Ramos (University)

Batista et al. (2023) The Conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein. J Mol Bio

Chebaiki et al. (2023) Discovery of new diaryl ether inhibitors against Mycobacterium tuberculosis targeting the minor portal of InhA. Eur J Med Chem

Ferré et al. (2023) Sodium is a negative allosteric regulator of the ghrelin receptor. Cell Rep

Bon et al. (2022) Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol

Boudehen et al. (2022) Mycobacterial resistance to zinc poisoning requires assembly of P-ATPase-containing membrane metal efflux platforms. Nat Commun

Le et al. (2022) Drug screening approach against mycobacterial fatty acyl-AMP ligase FAAL32 renews the interest of the salicylanilide pharmacophore in the fight against tuberculosis. Bioorg Med Chem

Mansour et al. (2022) Substrate recognition and cryo-EM structure of the ribosome-bound TAC toxin of Mycobacterium tuberculosis. Nat Commun

Tournier et al. (2020) An engineered PET depolymerase to break down and recycle plastic bottles. Nature

Crystal structure of a methyltransferase (HMA or MMA4) from M. tuberculosis in complex with S-adenosyl-N-decyl-aminoethyl (SADAE). ©Olivier Saurel

More about the facility: click here