Cellular biologist studying the actin cytoskeleton using advanced cell imaging methods. Podosomaholic.
Current position CNRS research engineer (IRHC) Co-PI, with Christel Vérollet, of the team Phagocyte architecture and dynamics. Institute of Pharmacology and Structural biology (IPBS), UMR 5089, CNRS-University of Toulouse, France ORCID | Twitter
I am a cell biologist working as a co-PI, with Christel Vérollet, of the team Phagocyte Architecture and Dynamics. I received my PhD in cell biology from Toulouse University in 2006. My dissertation work dealt with the formation of podosomes and the characterization of macrophage migration in tridimensional environments (Cougoule et al. 2010 Blood; Van Goethem et al. 2010 J. Immunol.; Poincloux et al. 2007 J. Cell Physiol.). I then joined Philippe Chavrier’s lab at the Curie Institute for two years, where I worked on the mechanisms involved in the formation of cancer cell invadopodia and on the mechanism used by breast cancer cells to invade 3D matrices (Hawkins et al. 2011 Biophys. J.; Poincloux et al. 2011 PNAS; Poincloux et al. J. Cell Science 2009 ; Lizarraga#, Poincloux# et al. 2010 Cancer Res.; Steffen et al. 2008 Curr. Biol.). In 2008, I was recruited as a permanent research engineer by CNRS to develop new imaging approaches dedicated to solve cell biology problems. With our team, we made an important contribution to the field of macrophage migration by demonstrating that macrophages have the unique capacity amongst leukocytes to use two different modes of migration depending on the architecture of the extracellular matrix, and we started revealing the role of podosomes in macrophage mesenchymal migration (Gui et al. 2018 Cancer Immunol. Res.; Vérollet et al. 2015 Blood; Guiet et al. 2012 J. Biol. Chem.). More recently, our lab pioneered the field of podosome mechanobiology, and designed the first and only method to evaluate podosome protrusion forces (Labernadie et al. 2014 Nat. Commun.; Proag et al. 2015 ACS Nano; Bouissou et al. 2017 ACS Nano ; Cervero et al. 2018 Nat Commun. ; Jasnin et al. 2022 Nat Commun.). My current main interests are still macrophage migration in general, and the mechanical functioning of podosomes in particular, but also the mechanical role of podosome–like structures involved during phagocytosis.
My scientific achievements consist in 50 research articles, accumulating >4300 citations with an h-index of 29, according to Google Scholar.
Selection of 5 publications
Jasnin M, Hervy J, Balor S, Bouissou A, Proag A, Voituriez R, Schneider J, Mangeat T, Maridonneau-Parini I, Baumeister W, Dmitrieff S, Poincloux R. Elasticity of podosome actin networs produces nanonewton forces. Nat Commun (2022)
Desvignes E, Bouissou A, Laborde A, Mangeat T, Proag A, Vieu C, Thibault C, Maridonneau-Parini I*, Poincloux R*. Nanoscale forces during confined cell migration. Nano Lett (2018)
Bouissou A#, Proag A#, Bourg N, Pingris K, Cabriel C, Balor S, Mangeat T, Thibault C, Vieu C, Dupuis G, Fort E, Lévêque-Fort S, Maridonneau-Parini I*, Poincloux R*. Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring. ACS Nano (2017)
Proag A#, Bouissou A#, Mangeat T, Voituriez R, Delobelle P, Thibault C, Vieu C, Maridonneau-Parini I*, Poincloux R*. Working together: Spatial synchrony in the force and actin dynamics of podosome first neighbors. ACS Nano (2015)
Labernadie A, Bouissou A, Delobelle P, Balor S, Voituriez R, Proag A, Fourquaux I, Thibault C, Vieu C, Poincloux R*, Charrière GM*, Maridonneau-Parini I*. Protrusion Force Microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes. Nat Commun (2014)