Responsable d'équipe
Notre équipe figure parmi les leaders mondiaux pour la caractérisation des vaisseaux sanguins HEV (high endothelial venules), portes d’entrée des lymphocytes dans les organes lymphoïdes, et les tissus enflammés et tumoraux, et pour l’étude de l’interleukine 33 (IL-33), une cytokine nucléaire de la famille IL-1 que nous avons découverte en 2003 et qui joue des rôles cruciaux dans l’immunité innée et l’inflammation allergique.
L'expertise unique et pluridisciplinaire de notre équipe est un atout majeur pour étudier les rôles des vaisseaux sanguins HEV et de l'IL-33 dans l'immunité, l'inflammation et le cancer.
Nous avons démontré que les cellules dendritiques, bien connues pour leur rôle de cellules présentatrices d’antigènes, jouent un rôle inattendu et important dans la régulation des vaisseaux sanguins HEV des ganglions lymphatiques (Nature 2011). En utilisant la technique de RNAseq sur cellule unique, nous avons identifié les gènes impliqués dans le dialogue entre les cellules endothéliales des vaisseaux HEV et les cellules dendritiques (Cell Rep 2019). Il y a une dizaine d’années, nous avons découvert la présence fréquente de vaisseaux sanguins de type HEV (TA-HEVs) dans les tumeurs solides humaines, et leur association avec l’infiltration de lymphocytes cytotoxiques et une évolution clinique favorable dans le cancer du sein (Cancer Res 2011, 311 citations). Notre étude a introduit un nouveau concept en cancérologie: “Les vaisseaux sanguins dans les tumeurs humaines ne sont pas tous identiques et certains types de vaisseaux trouvés dans le microenvironnement tumoral (vaisseaux TA-HEVs) peuvent contribuer à l’inhibition de la croissance des tumeurs plutôt qu’à leur développement”. Nous avons récemment démontré que les TA-HEVs permettent l’entrée des lymphocytes dans les tumeurs au cours des réponses immunitaires antitumorales et de l’immunothérapie contre le cancer, et sont associés à une meilleure réponse clinique des patients atteints de mélanome métastatique et traités par immunothérapie anticancéreuse anti-PD-1/anti-CTLA-4 (Cancer Cell 2022). Nous travaux suggèrent que de nouvelles stratégies thérapeutiques basées sur la modulation des vaisseaux TA-HEVs pourraient avoir un impact majeur sur l’immunité antitumorale et la réponse à l’immunothérapie des patients atteints de cancer.
L’IL-33 est une cytokine nucléaire de la famille IL-1 que nous avons initialement découverte comme un facteur nucléaire majeur des vaisseaux HEV (NF-HEV) (PNAS 2007, 756 citations). Nous avons observé que l’IL-33 est exprimée à des niveaux élevés dans les vaisseaux sanguins et les tissus des barrières épithéliales (PLoS 2008, 875 citations), et qu’elle fonctionne comme un signal d’alarme (alarmine) libéré lors de dommages cellulaires pour alerter les cellules immunitaires exprimant le récepteur de l’IL-33, ST2 (PNAS 2009, 510 citations). Ces cellules immunitaires incluent divers types de cellules impliquées dans l’immunité de type 2 et l’inflammation allergique telles que les mastocytes et les cellules lymphoïdes innées du groupe 2 (ILC2). Nous avons démontré que les protéases inflammatoires peuvent générer des formes tronquées d’IL-33 qui sont 30 fois plus puissantes que la protéine pleine taille pour l’activation des ILC2s (PNAS 2012, 389 citations, PNAS 2014). De plus, nous avons découvert que l’IL-33 pleine taille fonctionne comme un ‘senseur’ de protéase qui détecte les activités protéolytiques associées à une grande variété d’allergènes environnementaux (Nat Immunol 2018, 174 citations). Un objectif important de notre équipe est de mieux caractériser la régulation et les mécanismes d’action de l’IL-33 in vivo, grâce à l’utilisation d’approches multidisciplinaires.
Corinne Cayrol (CR HC, CNRS)
Jean-Philippe Girard (DR, CE Inserm)
Emma Lefrançais (CR CNRS)
Nathalie Ortéga (MCU, UPS)
Elisabeth Bellard (CNRS)
Pascale Mercier (CNRS)
Stéphane Roga (CNRS)
Clara Saint-Martin
Dorian Tarroux
Jade Hebras
Léa Fromont
Lucie Gelon
Jerko Ljubetic
Estefania Vina-Barrientos
Asrir*, Tardiveau*, Coudert*, Laffont*, Blanchard* et al. (2022) Tumor-associated high endothelial venules mediate lymphocyte entry into tumors and predict response to PD-1 plus CTLA-4 combination immunotherapy. Cancer Cell
Cayrol*, Duval* et al. (2018) Environmental allergens induce allergic inflammation through proteolytic maturation of IL-33. Nat Immunol
Lefrançais*, Duval* et al. (2014) Central domain of IL-33 is cleaved by mast cell proteases for potent activation of group-2 innate lymphoid cells. Proc Natl Acad Sci USA
Martinet*, Garido* et al. (2011) Human solid tumors contain high endothelial venules (HEV): association with T and B lymphocyte infiltration and favourable prognosis in breast cancer. Cancer Res
Moussion and Girard (2011) Dendritic cells control lymphocyte entry to lymph nodes via high endothelial venules. Nature
Cayrol and Girard (2009) The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1. Proc Natl Acad Sci USA
Specialist in the analysis of scientific production, Clarivate identifies each year the most influential researchers in the world. The “Highly Cited Researchers” are thus the researchers whose work is the most cited, typically those having several articles in the top 1% of citations in their field during the last decade in the “Web of science ™”.
This list is used in the famous ranking of universities in Shanghai.
Once again a member of the list of “Highly Cited Researchers” (2022), Jean-Philippe Girard, Inserm research director, head of the team “Vascular Biology: Endothelial cells in Immunity, Inflammation and Cancer” at the IPBS-Toulouse (CNRS-Université Paul Sabatier), is, for the third time, one of the most influential researchers in Immunology.
On December 17th in Paris, Dr. Corinne Cayrol received the Edouard Bonnefous Prize 2019 from the National Academy of Medicine for her work on Interleukin-33 (IL-33) as an important therapeutic target for asthma, regulated by environmental allergens.
This prize rewards all work on the environment and its consequences on human health.
Jean-Philippe GIRARD, Silver Medal of the CNRS 2013, Inserm Research Director at IPBS (CNRS-University of Toulouse), will receive the Prize Jean Valade 2018 from ‘Fondation de France’ on March 28th at the Collège de France in Paris, for his work on IL-33, a major therapeutic target for asthma and allergic diseases.
Jean-Philippe Girard is honored for his pioneering work on the discovery and characterization of IL-33, a protein that is now recognized as a central mediator of allergic inflammation and asthma. Asthma is one of the most common chronic inflammatory diseases in humans, affecting up to 300 million people worldwide. Progress in the prevention and treatment of asthma and allergic diseases is thus urgently needed. Identification and validation of novel therapeutic targets is critical in order to develop future treatments for those who do not respond to available therapies. The work of Jean-Philippe Girard and his team over the past 15 years has significantly contributed to the current knowledge about IL-33 mechanisms of action and regulation and the validation of IL-33 as a bona fide therapeutic target for asthma and other allergic diseases, such as atopic dermatitis.
Emma Lefrançais, CNRS Research associate in the laboratory of Jean-Philippe Girard, received one of the two “Grand Prix Jeune Chercheur” from the FONROGA Foundation.
Using intravital lung imaging, Emma’s work focuses on the role of IL-33 in inflammation and cellular dynamics during respiratory allergies.
The FONROGA Foundation supports initiatives and achievements in the fields of culture and health in the Toulouse region. Since its creation in 2016, more than €160,000 have been given to young researchers through Awards and fellowships. At IPBS, the Foundation has already given the “Grand Prix Jeune Chercheur” to three young researchers (Drs. Laure Gibot, Etienne Meunier and Emma Lefrançais).
Dr. Jean-Philippe GIRARD, received the Jean-Paul Binet Prize 2016 on December 5th, at the “Fondation pour la Recherche Médicale” in Paris.
This Prize rewards researchers who have made important advances in the understanding of the cardiovascular system. Jean-Philippe Girard received the prize for his work on HEV blood vessels and their role in inflammation and cancer.
Dr. Jean-Philippe GIRARD received the prize “Gallet et Breton” on December 17th 2013, at the National Academy of Medecine in Paris. This prestigious Prize in Cancerology rewards researchers who have made important advances in cancer research or cancer therapy. Jean-Philippe Girard received the prize for his work on HEV blood vessels and their role in cancer.
The CNRS Silver Medal honors researchers who are only at the beginning of their rise to fame, but who are already recognized nationally and internationally for the originality, quality, and importance of their work.
Jean-Philippe Girard, Director of IPBS, received this prestigious award for his work on the role of blood vessels in inflammation and cancer.
On October 16th 2012, Dr. Jean-Philippe Girard received the prestigious Prize in Cancerology, delivered every two years by the National Academy of Sciences and the ‘Institut de France’, for his work on HEV blood vessels and their role in cancer.
On 27 September 2012, Dr. Jean-Philippe GIRARD, Director of IPBS, received the first “Stars of the Health (Étoiles de la Santé) Cancer Research Prize” at the ’Cité de l’Espace’ in Toulouse, France.
The “Stars of the Health Prizes” were delivered for the first time this year by Foundation Oréade-Prévifrance. This event aimed at celebrating clinical, therapeutic and scientific research actors as well as start-up, pharmaceutical companies and associations working in the field of “Health and Medicine” in the south-west of France.
Five awards were delivered including “The Cancer Research Award” which rewards teams involved in fundamental or clinical research in the field of prevention and treatment of cancer. This award was given to Dr. Jean-Philippe Girard and his team for their work on HEV blood vessels in human cancer.
Dr. Girard receives the “”Stars of the Health Cancer Research Prize 2012″” at the ’Cité de l’Espace’ in Toulouse on September 27th (in French).
Dr. Jean-Philippe Girard, Director of IPBS and Head of the team “Vascular biology : endothelial cells, inflammation and cancer” is the recipient of the “Ruban Rose Avenir 2011 Prize”.
The“Ruban Rose Prizes” are awarded each year since 2004 to researchers who have made important contributions in breast cancer research.
The complete list of our publications is available through Pubmed
We went against the dogma that ‘blood vessels facilitate tumor growth’ and introduced a novel concept in cancer biology: ‘Blood vessels in human tumors are not all the same and some types of blood vessels (i. e. tumor-associated HEVs) may contribute to the fight against cancer’.
We showed that dendritic cells, which are well known for their role as antigen-presenting cells, play an unexpected and important role in the maintenance of HEV blood vessels in adult lymphoid tissues.
We found that high densities of tumor-associated HEVs are correlated with high levels of lymphocyte infiltration and favorable clinical characteristics in primary human melanomas.
We used intravital microscopy in mouse to study the in vivo trafficking in HEVs of human leukemic cells from chronic lymphocytic leukemia patients.
We reported the 1st characterization of the full-length HEV transcriptome by single cell RNA-sequencing, discovered the heterogeneity of HEV endothelial cells in homeostatic and inflamed lymph nodes and revealed important differences in the regulation of HEV genes after interruption of LTbR signaling.
Using intravital microscopy, we demonstrated that TA-HEVs are major sites of lymphocyte entry into tumors during anti-tumor immune responses and cancer immunotherapy. We also showed that TA-HEVs are associated with better clinical response and survival of metastatic melanoma patients treated with anti-PD-1/anti-CTLA-4 dual immunotherapy.
We were invited by the Nature Rev Immunol Chief Editor to write a comprehensive review on the role of HEV blood vessels in lymphocyte migration to lymph nodes.
We wrote a landmark review on the function and regulation of HEV blood vessels in immunity, inflammation and cancer (34 pages, 339 references).
We discovered a novel nuclear factor abundantly expressed in HEV endothelial cells in human lymphoid organs, that we designated nuclear factor of HEV (NF-HEV).
We showed that the IL-1-like cytokine IL-33 is a chromatin-associated cytokine, which is identical to NF-HEV, the nuclear factor that we cloned from purified HEV endothelial cells in 2003.
We reported that IL-33 is constitutively expressed in blood vessels and epithelial barrier tissues, and we proposed the concept that IL-33 may function as a novel alarmin (alarm signal) rather than as a classical cytokine (we were the first to propose this concept).
We discovered a unique example of molecular mimicry between a virus and a nuclear cytokine, and proposed that Kaposi’s sarcoma virus hijacked the chromatin-binding domain of IL-33 in order to maintain the viral genome in infected cells.
Contrary to the dogma in the field, we showed that IL-33 does not require maturation for biological activity and that cleavage by caspases results in IL-33 inactivation, rather than activation (We were the first to report these findings, which were later confirmed by 3 other teams).
We showed that processing of IL-33 by inflammatory proteases generate mature forms of the cytokine with highly increased biological activity.
We reported the atlas of IL-33 protein expression and Il33 promoter activity in mouse embryos and adult tissues at steady state and during inflammation.
We discovered that cleavage within the central domain of IL-33 generates truncated forms that are 30-fold more potent than the native protein for activation of ILC2s, the major targets of IL-33 in vivo.
We discovered that IL-33 functions as an allergen protease sensor that detects proteolytic activities associated with various clinically relevant aeroallergens and initiates allergic inflammation shortly after allergen exposure.
We were invited to write a state of the art review on IL-33 mode of action, regulation and functions in health and disease.
We were invited to write a state of the art review on IL-33 protein and its roles in immunity and allergic inflammation.
We were invited to write a state of the art review on IL-33 mode of action and regulation.
We were invited to make a critical review of IL-33 biology. Importantly, we alerted the scientific community to the problems of specificity of IL-33 reagents, and we made recommendations to generate reliable results.
Title : NOVEL SUPERACTIVE IL-33 FRAGMENTS, AND USES THEREOF
Publication N° WO/2012/113927
Publication Date: 30.08.2012
International Application No PCT/EP2012/053199
Applicants: CNRS
Inventors: GIRARD, Jean-Philippe;CAYROL-GIRARD, Corinne; LEFRANCAIS, Emma